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PE anti-mouse CD130 (gp130) Antibody

Pricing & Availability
Clone
4H1B35 (See other available formats)
Regulatory Status
RUO
Other Names
GP130, IL6ST, IL-6RB, IL-6R-beta, Oncostatin-M receptor subunit alpha, IL-6 receptor Beta
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
4H1B35_PE_CD130_Antibody_1_121715
C57BL/6 mouse splenocytes were stained with CD3 FITC and CD130 (clone 4H1B35) PE (top) or rat IgG2b, κ PE isotype control (bottom).
  • 4H1B35_PE_CD130_Antibody_1_121715
    C57BL/6 mouse splenocytes were stained with CD3 FITC and CD130 (clone 4H1B35) PE (top) or rat IgG2b, κ PE isotype control (bottom).
  • 4H1B35_PE_CD130_Antibody_2_121715
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149403 25 µg 117 CHF
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149404 100 µg 287 CHF
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Description

CD130, also known as gp130 and IL-6ST, is a type I transmembrane glycoprotein. CD130 can form receptor complexes for a family of cytokines, which include IL-6, IL-11, IL-27, LIF, and OSM, and can transduce signals for these cytokines. The 597aa extracellular domain has one Ig-like domain and five fibronectin type-III repeats. The 277a.a. intracellular domain does not have intrinsic kinase activity. Instead, it can be phosphorylated at tyrosine residues after it forms complexes with other receptor subunits, and in turn can lead to the association with JAK tyrosine kinases and the activation of STAT transcription factors, particularly STAT3.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD130 (a.a. 23-619) expressed in 293E cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. van Oostrum M, et al. 2019. Nat Commun. 4.398611111. PubMed
  2. Ng S, et al. 2022. Sci Rep. 12:14087. PubMed
RRID
AB_2566644 (BioLegend Cat. No. 149403)
AB_2566645 (BioLegend Cat. No. 149404)

Antigen Details

Structure
Single pass type 1 membrane glycoprotein.
Distribution

T cells, activated B cells, plasma cells, and monocytes.

Function
Signal transduction.
Interaction
IL-6RA, IL-11RA, IL-27RA, OSMR, LIFR, CNTFR, JAK1, and SOCS3.
Cell Type
B cells, Dendritic cells, Monocytes, Plasma cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Hibi M, et al. 1990. Cell 63:1149.
2. Kishimoto T, et al. 1995. Blood 86:1243.

Gene ID
16195 View all products for this Gene ID
UniProt
View information about CD130 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 12.18.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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