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PE anti-mouse CD117 (c-kit) Antibody

Pricing & Availability
Clone
S18020H (See other available formats)
Regulatory Status
RUO
Other Names
SCO1, SOW3, CD117, Tr-kitm, Gsfsco5, c-kit
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
S18020H_PE_CD117_c-kit_Antibody_062321.png
C57BL/6 mouse bone marrow cells were stained with anti-mouse CD11b APC and anti-mouse CD117 (clone S18020H) PE (left) or anti-rat IgG1, κ PE isotype control (right). Data shown were gated on total cell population.
  • S18020H_PE_CD117_c-kit_Antibody_062321.png
    C57BL/6 mouse bone marrow cells were stained with anti-mouse CD11b APC and anti-mouse CD117 (clone S18020H) PE (left) or anti-rat IgG1, κ PE isotype control (right). Data shown were gated on total cell population.
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161605 25 µg 73 CHF
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161606 100 µg 214 CHF
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Description

CD117 is a 145 kD immunoglobulin superfamily member also known as c-kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse c-kit transfectants
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

S18020H has been tested to block the ligand binding of SCF on c-kit which is determined by the phosphorylation of ERK1/2 in c-kit signaling.

RRID
AB_2894652 (BioLegend Cat. No. 161605)
AB_2894652 (BioLegend Cat. No. 161606)

Antigen Details

Structure
A 145 kD immunoglobulin superfamily member
Distribution

Hematopoietic stem cells, mast cells, and some acute myeloid leukemia cells

Function
The development of hepatopoietic stem cells
Ligand/Receptor
Steel factor, stem cell factor, and mast cell growth factor
Antigen References
  1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
  2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
  3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
  4. Besmer P, et al. 1986. Nature. 320:415.
  5. Witte ON. 1990. Cell. 63:5.
Gene ID
16590 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 06.23.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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