PE anti-mouse CD103 Antibody

Pricing & Availability
Clone
2E7 (See other available formats)
Regulatory Status
RUO
Other Names
Integrin αIEL chain, Integrin αE chain, αE integrin, ITGAE
Isotype
Armenian Hamster IgG
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Product Citations
publications
2E7_PE_081908.jpg
C57BL/6 splenocytes stained with 2E7 PE
  • 2E7_PE_081908.jpg
    C57BL/6 splenocytes stained with 2E7 PE
See PE spectral data
Cat # Size Price Quantity Check Availability Save
121405 25 µg 60,00 CHF
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121406 100 µg 180,00 CHF
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Description

CD103 is a type I transmembrane glycoprotein known as αE integrin or Integrin αIEL chain. It belongs to the integrin family and is primarily found on intestinal intraepithelial lymphocytes (IEL). CD103 is also expressed on a subpopulation of lamina propria T cells, epithelial dendritic cells, lamina propria-derived dendritic cells, and a small subset of peripheral lymphocytes. T regulatory cells express high level of CD103. The CD103 expression on lymphocytes can be induced upon activation and TGF-β stimulation. In association with integrin β7, CD103 is expressed as αE/β7 heterodimer. Mature CD103 protein can be cleaved into 2 chains, a 150 kD (C-terminal) chain and a 25 kD (N-terminal) chain, which remain linked by disulfide bonds. CD103 binds to E-cadherin and mediates homing of lymphocytes to the intestinal epithelium.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse intestinal intraepithelial lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining1,7 of acetone-fixed frozen sections, immunofluorescence2, and in vitro activation1.

Application References

(PubMed link indicates BioLegend citation)
  1. LeFrancois L, et. al, 1994. Eur. J. Immunol. 24:635. (FC, IHC, IP)
  2. Mysorekar IU, et. al, 2002. J. Biol. Chem. 277:37811. (FC, IF)
  3. Mikami N, et al. 2011. J. Immunol. 186:6886. PubMed
  4. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  5. Quinn KM, et al. 2013. J. Immunol. 191:5085. PubMed
  6. Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed
  7. Xiao B, et al. 2015. PLoS One 1:e0115333. (IHC)
Product Citations
  1. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
  2. Beura LK, et al. 2018. Immunity. 48:327. PubMed
  3. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
  4. Van Braeckel-Budimir N, et al. 2018. Cell Rep. 24:3374. PubMed
  5. Ramakrishna C, et al. 2019. Nat Commun. 10:2153. PubMed
  6. Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
  7. Katagiri T, et al. 2019. Mucosal Immunol. 12:p1104. PubMed
  8. Hartwig T et al. 2018. Cell reports. 25(13):3564-3572 . PubMed
  9. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  10. Garo LP, et al. 2019. Cell Rep. 28:3353. PubMed
  11. Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
  12. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  13. Vogel AJ et al. 2015. Frontiers in immunology. 0.477083333 . PubMed
  14. Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
  15. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  16. Kyburz A, et al. 2019. J Allergy Clin Immunol. 143:1496. PubMed
  17. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  18. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  19. Jin J, et al. 2020. Cell Reports. 30(12):4124-4136. PubMed
  20. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  21. Parsons M, et al. 2014. J Immunol. 192:1361. PubMed
  22. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
  23. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  24. Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
  25. Yamazaki S, et al. 2015. J Immunol. 195: 3427 - 3435. PubMed
  26. Rezende R, et al. 2015. Nat Commun. 6: 8726. PubMed
  27. Wan X, Thomas J, Unanue E 2016. J Exp Med. 213: 967 - 978. PubMed
  28. Chandra J, et al. 2016. Immunol Cell Biol. 10.1038/icb.2016.83. PubMed
  29. Ahadome S, et al. 2016. JCI Insight. 1:e87012. PubMed
  30. Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
  31. Guo S, et al. 2021. Frontiers in Immunology. 11:614667. PubMed
  32. Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed
  33. Kim S, et al. 2020. Immunity. 53(4):759-774.e9. PubMed
  34. Gonalves S, et al. 2021. Cell Reports. 34(11):108860. PubMed
  35. Keerthivasan S, et al. 2021. Immunity. 54(7):1511-1526.e8. PubMed
  36. Bruand M, et al. 2021. Cell Reports. 36(3):109412. PubMed
  37. Tian T, et al. 2020. Cancer Immunol Res. 660:8. PubMed
  38. Devalaraja S, et al. 2020. Cell. 1098:180. PubMed
  39. Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed
  40. Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed
  41. Bonavita E, et al. 2020. Immunity. 1215:53. PubMed
  42. Zhang XW, et al. 2021. Oncoimmunology. 10:1920739. PubMed
  43. Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed
  44. Tuong ZK, et al. 2021. iScience. 24:103326. PubMed
RRID
AB_535948 (BioLegend Cat. No. 121405)
AB_1133989 (BioLegend Cat. No. 121406)

Antigen Details

Structure
Type I transmembrane glycoprotein, Integrin family, can be cleaved into 150 kD and 25 kD chains, associated with β7 integrin
Distribution

Majority of intestinal intraepithelial lymphocytes (IEL), subpopulation of lamina propria T cells, epithelial dendritic cells, small subset of peripheral lymphocytes, Treg cells.

Function
Retention and activation of CD103+ lymphocytes in the intestinal epithelium, regulate tissue-specific T cell homing.
Ligand/Receptor
E-Cadherin
Cell Type
Dendritic cells, Lymphocytes, T cells, Tregs
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Kilshaw PJ and SJ. Murant. 1990. Eur. J. Immunol. 20:2201.
2. Karecla PI, et al. 1995. Eur. J. Immunol. 25:852.
3. LeFrancois L, et al. 1994. Eur. J. Immunol. 24:635.
4. Sung SS, et al. 2006. J. Immunol. 176:2161.
5. Johansson-Lindbom B, et al. 2005. J. Exp. Med. 202:1063.
6. Dujardin HC, et al. 2004. Proc. Natl. Acad. Sci. USA. 101:14473.

Gene ID
16407 View all products for this Gene ID
UniProt
View information about CD103 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 05.04.2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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