PE anti-IRF6 Antibody

Pricing & Availability
Clone
14B2C16 (See other available formats)
Other Names
Interferon regulatory factor 6, IRF-6
Isotype
Mouse IgG2a, κ
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Product Citations
publications
14B2C16_PE_IRF6_Antibody_050616
HaCat cells were treated with Fixation Buffer and permeabilized with True-Phos™ Perm Buffer, then stained with anti-IRF6 (clone 14B2C16) PE (filled histogram) or mouse IgG2a, κ PE (open histogram).
  • 14B2C16_PE_IRF6_Antibody_050616
    HaCat cells were treated with Fixation Buffer and permeabilized with True-Phos™ Perm Buffer, then stained with anti-IRF6 (clone 14B2C16) PE (filled histogram) or mouse IgG2a, κ PE (open histogram).
See PE spectral data
Cat # Size Price Quantity Avail. Save
674503 25 tests 135 CHF
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674504 100 tests 325 CHF
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Description

IRF6 is a member of the IRF family of transcription factors, which are based on the presence of the conserved IRF tryptophan pentad repeat DNA-binding domain. Unlike other IRF transcription factors, IRF6 is not involved in the expression of interferon genes and doesn’t seem to be involved in innate immunity. Data suggests that IRF6 is necessary for appropriate epidermal differentiation and development. Mutations of IRF6 are associated with human craniofacial developmental defects such as cleft lip, palate, tooth agenesis, Van der Woude syndrome, and popliteal pterygium syndrome. IRF6-deficient mice display severe morphological defects in skin and limbs, and result in perinatal lethality as well.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human IRF6 recombinant protein (92-223 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2616776 (BioLegend Cat. No. 674503)
AB_2616777 (BioLegend Cat. No. 674504)

Antigen Details

Structure
467 amino acids with a predicted molecular weight of 53 kD and contains an IRF trytophan pentad repeat that is responsible for DNA binding.
Distribution
Nucleus and cytoplasm.
Function
IRF6 is a transcriptional activator that regulates proliferation and differentiation of both epithelial cells and keratinocytes. IRF6 is required for normal epidermal development during embryogenesis.
Interaction
Interacts with SERPINB5.
Biology Area
Cell Biology, Transcription Factors
Antigen References

1. Melath A, et al. 2013. Asian Pac. J. Cancer Prev. 14:6803.
2. Salahshourifar I, et al. 2012. Eur. J. Med. Genet. 55:389.
3. Blackburn J, et al. 2012. Dev. Biol. 365:61.
4. Botti E, et al. 2011. Proc. Natl. Acad. Sci. USA 108:13710.
5. Restivo G, et al. 2011. EMBO 30:4571.
6. Fleming JA, et al. 2009. Mol. Cell Endocrinol. 299:252.

Gene ID
3664 View all products for this Gene ID
UniProt
View information about IRF6 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 05.13.2016

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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