PE anti-human TNAP Antibody

Pricing & Availability
W8B2 (See other available formats)
Other Names
Tissue Non-specific Alkaline Phosphatase, mesenchymal stem cell antigen-1, MSC, W8B2
Mouse IgG1, κ
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Product Citations
Human retinoblastoma cell line Weri-RB cells stained W8B2 PE
  • W8B2_PE_021808
    Human retinoblastoma cell line Weri-RB cells stained W8B2 PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
327305 25 tests 120 CHF
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327306 100 tests 260 CHF
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The W8B2 antibody reacts with an antigen, TNAP (Tissue non-specific alkaline phosphatase), that is selectively expressed on bone marrow mesenchymal stem cells, but not on placenta-derived mesenchymal stem cells. It was previously reported that the antibody recognized MSCA-1, mesenchymal stem cell antigen-1. Mesenchymal stem cells or MSCs were originally named as bone marrow stromal cells. They are a population of adult stem cells with a large capacity for self-renewal and multipotency for differentiation into a variety of cell types including osteoblasts, chondrocytes, myocytes, adipocytes, β-pancreatic islets cells and certain neuronal cells. MSCs reside in many tissues, such as bone marrow, placenta, adipose tissue, adult peripheral blood, fetal blood, skin, as well as liver and lung. Besides their plasticity for tissue repair, MSCs also exhibit a powerful immunosuppressive activity and play important role in supporting hematopoiesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Human, African Green, Baboon, Cynomolgus
Antibody Type
Host Species
Retinoblastoma cell line (Weri-RB-1 cells)
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Battula VL, et al. 2009. Haematologica. 94:173.
  2. Aomatsu E, et al. 2014. Sci Rep. 4:3652. PubMed
Product Citations
  1. Aomatsu E, et al. 2014. Sci Rep. 4:3652. PubMed
AB_940677 (BioLegend Cat. No. 327305)
AB_940679 (BioLegend Cat. No. 327306)

Antigen Details


Bone marrow mesenchymal stem cells

Cell Type
Mesenchymal cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Antigen References

1. Battula VL, et al. 2007. Differentiation. 75:279.

Gene ID
249 View all products for this Gene ID
View information about TNAP on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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