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PE anti-human CD53 Antibody

Pricing & Availability
Clone
HI29 (See other available formats)
Regulatory Status
RUO
Workshop
IV N18
Other Names
OX44, MOX44
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HI29_PE_021009.jpg
Human peripheral blood lymphocytes stained with HI29 PE
  • HI29_PE_021009.jpg
    Human peripheral blood lymphocytes stained with HI29 PE
See PE spectral data
Cat # Size Price Quantity Check Availability Save
325406 100 tests 281 CHF
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Description

CD53 is a 35-42 kD type III tetraspan membrane protein. It is expressed on all leukocytes, but not on platelets or erythrocytes. CD53 is thought to be involved in signal transduction. It has been shown to interact with a number of proteins including IL-4, CD20, CD2, CD9, IL-2, VLA-4, and CD4. Cross-linking of CD53 promotes B cell activation. The CD53 antigen is highly glycosylated and treatment with endoglycosidase F reduces the apparent molecular weight of this protein by approximately 25 kD. The HI29 antibody has been shown to be useful for flow cytometry, and immunohistochemistry (frozen).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Mollinedo F, et al. 1997. Clin. and Diag. lab. Immunol. 4:229.
  2. Cao L, et al. 1997. Immunobiology 197(1):70.
  3. Amiot M. 1990. J. Immunol. 145:4322.
  4. Vanherberghen B, et al. 2004. Proc Natl Acad Sci USA. 101(48):16873.
  5. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Product Citations
  1. Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed
RRID
AB_2075725 (BioLegend Cat. No. 325406)

Antigen Details

Structure
Cell surface protein, tetraspan family, four membrane spanning hydrophobic domains, 35-42 kD
Distribution

Hematopoietic cells including neutrophils, monocytes, B cells, T cells (single positive thymocytes and peripheral T cells), and eosinophils. Not expressed on platelets, erythrocytes, and non-hematopoietic cells

Function
Signal transduction, cross-linking on B cells causes activation
Interaction
IL-4, CD20, CD2, CD9, IL-2, integrin α4, integrin β1, CD4
Modification
Heavily glycosylated, treatment with endoglycosidase F reduces apparent molecular weight by 25 kD
Cell Type
B cells, Eosinophils, Neutrophils
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Amiot M. 1990. J. Immunol. 145:4322.
2. Angelisova P, et al. 1990. Immunogenetics 32:281.
3. Olweus J, et al. 1993. J. Immunol. 153:4997.
4. Leukocyte Typing IV. Knapp W, et al. (Eds) Oxford University Press (1989)

Gene ID
963 View all products for this Gene ID
UniProt
View information about CD53 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 12.15.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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