PE anti-human CD100 Antibody

Pricing & Availability
A8 (See other available formats)
Regulatory Status
Other Names
Mouse IgG1, κ
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Product Citations
Human peripheral blood lymphocytes stained with A8 PE
  • A8_PE_090808.jpg
    Human peripheral blood lymphocytes stained with A8 PE
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328407 25 tests 100,00 CHF
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328408 100 tests 220,00 CHF
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CD100, a 150 kD homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Host Species
Human PHA activated lymphocytes
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Hall K, et al. 1996. P. Natl. Acad. Sci. USA 93:11780.
  2. Mizrahi S, et al. 2007. PLoS One. 2(9):e818.
  3. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
AB_2285709 (BioLegend Cat. No. 328407)
AB_2301554 (BioLegend Cat. No. 328408)

Antigen Details

Class IV semaphorin family member, 150 kD homodimer
T cells, B cells, weak on NK cells
Costimulatory molecule in adaptive immune responses that switches off negative signaling from CD72
Cell Type
T cells, B cells, NK cells
Biology Area
Molecular Family
CD Molecules
Antigen References

1. Schlossman SL Bloumsell W Gilks et al. eds. 1995. Leucocyte Typing V:White Cell Differentiation Antigens. Oxford University Press New York.
2. Bougeret CIG Mansur H Dastot et al. 1992. Increased surface expression of a newly identified 150 kDa dimer early afterhuman T lymphocyte activation. J. Immunol. 148:318.
3. Knapp WB Dorken EP. Rieber et al, eds. 1989. Leucocyte Typing IV:White Cell Differentiation Antigens. Oxford University Press New York.

Gene ID
10507 View all products for this Gene ID
View information about CD100 on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD100

  • FITC anti-human CD100

  • PE anti-human CD100

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