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PE anti-β Catenin 1 (CTNNB1) Antibody

Pricing & Availability
Clone
12F7 (See other available formats)
Regulatory Status
RUO
Other Names
Catenin (Cadherin-Associated Protein), Beta 1, 88 kD, CTNNB1, TNNB, MRD19, armadillo, catenin beta-1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
12F7_PE_Catenin-1_Antibody_020720
Human colon adenocarcinoma cell line SW480 treated (open historgram) or untreated (filled histogram) with tankyrase inhibitor XAV939 for 18 hours, then treated with True-Nuclear™ Transcription Factor Buffer set (Cat. No. 424401) and stained with anti-β Catenin 1 (clone 12F7) PE.
  • 12F7_PE_Catenin-1_Antibody_020720
    Human colon adenocarcinoma cell line SW480 treated (open historgram) or untreated (filled histogram) with tankyrase inhibitor XAV939 for 18 hours, then treated with True-Nuclear™ Transcription Factor Buffer set (Cat. No. 424401) and stained with anti-β Catenin 1 (clone 12F7) PE.
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844605 25 tests 171 CHF
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844606 100 tests 384 CHF
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Description

β-catenin is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. β-catenin also plays a key role in Wnt signaling pathways and thus is involved in neural differentiation, synaptic plasticity, neurodegenerative disease, and prevention of apoptosis. Finally, this protein binds to the product of the adenomatous polyposis coli (APC) gene, which is mutated in the adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant β-catenin (N-terminal fragment) fused to MBP.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol.

Application Notes

Additional reported applications (for the relevant formats) include: immunfluorescence microscopy4 and immunohistochemical staining3.

For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 844603) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Additional Product Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Sacco P, et al. 1995. J. Biol. Chem. 270:20201. (WB)
  2. Johnson KR, et al. 1993. Exp. Cell Res. 207:252.
  3. Gupta K, et al. 2012. J. Ped. Hem. Onc. 34:320. (IHC-P)
  4. Radice G, et al. 1997. Dev. Bio. 181:64. (IHC-P)
RRID
AB_2832858 (BioLegend Cat. No. 844605)
AB_2832859 (BioLegend Cat. No. 844606)

Antigen Details

Structure
88 kD armadillo repeat containing protein with an unstructured N and C terminal.
Distribution

β-catenin localizes to cell-cell contacts (adherens junctions) and the cell membrane as well as the cytoplasmic pool of protein. β-catenin can also translocate to the nucleus and is in interphase found in puncta at the proximal end of centrioles. In mitosis, it localizes to the centrosomes at the spindle.

Function
Wnt signaling pathway, regulation of cell-cell adhesion, neurogenesis, transcription, transcription regulation, early embryonic patterning, asymmetric cell division, stem cell renewal, and the epithelial to mesenchymal transition.
Interaction
Wnt signaling pathway, apoptotic cleavage of cellular proteins, and EphB-EphrinB signaling.
Biology Area
Cell Adhesion, Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Adhesion Molecules
Antigen References

1. Hirano S, et al. 2012. Physiol. Rev. 92:597.

Gene ID
1499 View all products for this Gene ID
UniProt
View information about beta Catenin 1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 02.06.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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