PE anti-BATF Antibody

Pricing & Availability
Clone
9B5A13 (See other available formats)
Other Names
B-cell-activating transcription factor, Basic leucine zipper transcriptional factor ATF-like, SFA-2, BATF1
Isotype
Mouse IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
9B5A13_PE_BATF_Antibody_FC_051414.jpg
CD3+ CD28- stimulated (3 days) human peripheral blood lymphocytes were treated with FOXP3 Fix/Perm Buffer Set and then intracellularly stained with BATF (clone 9B5A13) PE (filled histogram). Open histogram represents staining with non-stimulated cells.
  • 9B5A13_PE_BATF_Antibody_FC_051414.jpg
    CD3+ CD28- stimulated (3 days) human peripheral blood lymphocytes were treated with FOXP3 Fix/Perm Buffer Set and then intracellularly stained with BATF (clone 9B5A13) PE (filled histogram). Open histogram represents staining with non-stimulated cells.
See PE spectral data
Cat # Size Price Quantity Avail. Save
654803 25 tests 125 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
654804 100 tests 315 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

BATF is a basic leucine zipper transcription factor belonging to the ATF superfamily. BATF forms heterodimers with Jun proteins, which in turn binds to AP-1 consensus sites. The BATF/Jun heterodimers have significantly reduced transcriptional activity compared to Fos/Jun heterodimers. Therefore, induction of BATF expression suppresses AP-1 target genes by competing for Fos/Jun binding sequences. BATF is expressed at low levels in hematopoietic cells and can be induced in response to a variety of stimuli. BATF knockout mice show defects in cytokine expression and in the differentiation of T helper cell subsets and B cells, but not in the proliferation of lymphocytes. These findings suggest that BATF is a tissue-specific regulator for AP-1 activity and plays an essential role in the regulation of immune responses. During early EBV infection, BATF expression is induced by EBNA2 proteins, subsequently preventing infected B cells from entering apoptotic and lytic pathways. This indicates that BATF functions as a modulator to maintain latency status of virus-infected cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length human BATF recombinant protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Pham D, et al. 2019. Cell Rep. 29:1203. PubMed
RRID
AB_2563516 (BioLegend Cat. No. 654803)
AB_2563517 (BioLegend Cat. No. 654804)

Antigen Details

Structure
14 kD protein containing a bZIP (basic-leucine zipper) domain, which mediates dimerization with Jun family members.
Distribution

Nucleus. Expressed most abundantly in hematopoietic tissues.

Function
AP-1 transcription complexes regulate gene expression in response to various stimuli, such as cytokines, growth factors, stress, and infection. BATF belongs to the AP-1/ATF superfamily and negatively regulates AP-1 mediated transcription when it forms heterodimers with Jun proteins.
Interaction
Forms heterodimers with c-Jun, JunB, and JunD. Interacts with IFI35, IRF4, and IRF8 through bZIP domain.
Cell Type
B cells, Dendritic cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Betz BC, et al. 2010. J. Exp. Med. 207:933.
2. Dorsey MJ, et al. 1995. Oncogene 11:2255.
3. Echlin DR, et al. 2000. Oncogene 19:1752.
4. Johansen LM, et al. 2003. J. Virol. 77:6029.
5. Schraml BU, et al. 2009. Nature 460:405.
6. Tussiwand R, et al. 2012. Nature 490:502.

Gene ID
10538 View all products for this Gene ID
UniProt
View information about BATF on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 05.28.2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account