Pacific Blue™ anti-mouse CD48 Antibody

Pricing & Availability
Clone
HM48-1 (See other available formats)
Regulatory Status
RUO
Other Names
BCM1, SLAMF2, Blast-1
Isotype
Armenian Hamster IgG
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Product Citations
publications
HM48-1_Blue_091207
C57BL/6 mouse splenocytes stained with Pacific blue™ HM48-1
  • HM48-1_Blue_091207
    C57BL/6 mouse splenocytes stained with Pacific blue™ HM48-1
See Pacific Blue™ spectral data
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103417 25 µg 85,00 CHF
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103418 100 µg 195,00 CHF
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Description

CD48 is a 45 kD GPI-anchored glycoprotein also known as BCM1, Blast-1 (human), and OX-45 (rat). It is a member of the Ig superfamily, expressed on T and B cells and monocytes/macrophages. It plays a role in adhesion and T cell recognition. The primary ligands for CD48 are CD2 and CD244.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse T lymphoma MBL-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

The HM48-1 antibody is useful for blocking in vitro and in vivo CD48 mediated interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation1, costimulation of T cell proliferation1,2, blocking of CD48-CD2 interaction1, and inhibition of CTL activity and graft rejection1,2. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. Nos. 103452-103456) with a lower endotoxin limit (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Kato K, et al. 1992. J. Exp. Med. 176:1241. (IP, Costim, Block)
  2. Qin L, et al. 1994. J. Exp. Med. 179:341. (Costim, Block)
Product Citations
  1. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  2. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  3. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  4. Zhao M, et al. 2019. Cell Rep. 26:652. PubMed
  5. Li X, et al. 2019. Stem Cells. 37:937. PubMed
  6. Zhang B, et al. 2018. Nat Med. 24:450. PubMed
  7. Cabezas–Wallscheid N, et al. 2017. Cell. 169:807. PubMed
  8. Park DS, et al. 2019. PLoS Comput Biol. 15:e1006913. PubMed
  9. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  10. Davis FM, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2353. PubMed
  11. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  12. Nacson J, et al. 2020. Molecular Cell. 78(5):951-959. PubMed
  13. Wilkinson AC, et al. 2019. Nature. 571:117. PubMed
  14. Sauer A, et al. 2009. Blood. 114:3216. PubMed
  15. MacNamara K, et al. 2011. PLoS One. 6:e28669. PubMed
  16. Kang H, et al. 2012. J Immunol. 188:5970. PubMed
  17. Grassinger J, et al. 2012. Cytokine. 58:218. PubMed
  18. Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
  19. Nestorowa S, et al. 2016. Blood. 128: e20 - e31. PubMed
  20. Vannini N, et al. 2016. Nat Commun. 7:13125. PubMed
  21. Kang YA, et al. 2020. J Exp Med. 217:00:00. PubMed
  22. Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed
RRID
AB_756139 (BioLegend Cat. No. 103417)
AB_756140 (BioLegend Cat. No. 103418)

Antigen Details

Structure
Ig superfamily, 45 kD
Distribution

T cells and B cells, monocytes/macrophages

Function
Adhesion, T cell recognition
Ligand/Receptor
CD2, CD244
Cell Type
B cells, Macrophages, Monocytes, T cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Flament C, et al. 1996. Hum. Immunol. 46:82.
3. Van der Merwe PA, et al. 1995. Curr. Biol. 5:74.
4. Latchman Y, et al. 1998. J. Immunol. 161:5809.

Gene ID
12506 View all products for this Gene ID
UniProt
View information about CD48 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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