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Pacific Blue™ anti-human Lineage Cocktail (CD3, CD14, CD16, CD19, CD20, CD56)

Pricing & Availability
Clone
UCHT1; HCD14; 3G8; HIB19; 2H7; HCD56;
Regulatory Status
RUO
Other Names
Lin 1
Isotype
Mouse IgG1, κ/Mouse IgG2b, κ
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Product Citations
publications
PB_Human_Lineage_cocktail_Antibody_FC_1_101912
Human peripheral blood leukocytes were stained with Pacific Blue™ anti-Human Lineage Cocktail and HLA-DR PerCP.
  • PB_Human_Lineage_cocktail_Antibody_FC_1_101912
    Human peripheral blood leukocytes were stained with Pacific Blue™ anti-Human Lineage Cocktail and HLA-DR PerCP.
  • PB_Human_Lineage_cocktail_Antibody_FC_2_101912
    Human peripheral blood leukocytes co-stained with CD123 PE and HLA-DR PerCP (gated on the Lineage negative/dim cell population).
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348805 50 tests 698 CHF
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Description

This anti-Human Lineage Cocktail is optimized for the detection of human peripheral blood T cells, B cells, NK cells, monocytes, and neutrophils. In combination with other markers, it can be used for studies of dendritic cells and/or basophils. This cocktail is composed of CD3, CD14, CD16, CD19, CD20, and CD56. CD3 is the antigen mainly found on T cells; CD14 is expressed on monocytes/macrophages and at low levels on neutrophils and eosinophils; CD16 is expressed on NK cells, activated monocytes/macrophages, and neutrophils; CD19 and CD20 are on B cells; CD56 is located on NK cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules. New Jersey. (FC)
Product Citations
  1. Wang X, et al. 2021. Cell Discovery. 7(1):60. PubMed
  2. Hu EY, et al. 2020. JCI Insight. 5:00. PubMed
  3. Schielke L, et al. 2022. Front Immunol. 13:916701. PubMed
  4. Moravcikova E, et al. 2018. Cytometry A. 93:894. PubMed
  5. Saygin C, et al. 2021. Leukemia. 35:3406. PubMed
  6. Verma M, et al. 2021. J Exp Med. 218:. PubMed
RRID
AB_2889063 (BioLegend Cat. No. 348805)

Antigen Details

Distribution

T cells, B cells, NK cells, monocytes/macrophages, neutrophils

Cell Type
B cells, Dendritic cells, Macrophages, Mesenchymal Stem Cells, Monocytes, Neutrophils, NK cells, T cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Gene ID
915 View all products for this Gene ID 2214 View all products for this Gene ID 930 View all products for this Gene ID 931 View all products for this Gene ID 4684 View all products for this Gene ID 929 View all products for this Gene ID
UniProt
View information about Lineage on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 07.09.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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