ELISA MAX™ Deluxe Set Mouse TNF-α

Pricing & Availability
Regulatory Status
Other Names
Ave. Rating
Submit a Review
Product Citations
mouse tnf-a_122109
  • mouse tnf-a_122109
Cat # Size Price Quantity Check Availability Save
430904 5 plates 286 CHF
Check Availability

Need larger quantities of this item?
Request Bulk Quote
430915 10 Plates 550 CHF
Check Availability

Need larger quantities of this item?
Request Bulk Quote
430916 20 Plates 880 CHF
Check Availability

Need larger quantities of this item?
Request Bulk Quote

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price!

BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Mouse TNF-α ELISA MAX™ Capture Antibody (200X)
  • Mouse TNF-α ELISA MAX™ Detection Antibody (200X)
  • Mouse TNF-α Standard
  • Avidin-HRP (1000X)
  • Substrate Solution A
  • Substrate Solution B
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)

Product Details

Verified Reactivity


As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application References

(PubMed link indicates BioLegend citation)
  1. Patole PS, et al. 2005. J. Am. Soc. Nephrol. 16:3273.
  2. ChavantF, et al. 2010. J. Pharmacol Exp. Ther. 332:505. PubMed
  3. Lewis CJ, et al. 2010. J. Immunol. 184:3789. PubMed
  4. Qui J, et al. Appl Environ Microbiol. PubMed
  5. Yang HJ, et al. 2011. J Cell Sci. 124:2552. PubMed.
  6. White LE, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 303:449. PubMed
  7. Zhang X, et al. 2014. PLoS One. 9:90856. PubMed
  8. Zachman AL, et al. 2014. Biomaterials. 35:9635. PubMed
  9. Goldberg R, et al. 2014. Diabetes. 63:4302. PubMed
  10. Zahman AL, et al. 2014. Biomaterials. 35:9635. PubMed
  11. Justice JN, et al. 2015. J appl Physiol. 118:163. PubMed
Product Citations
  1. White L, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 303:449. PubMed
  2. Zhang X, et al. 2014. PLoS One. 9:90856. PubMed
  3. Goldberg R, et al. 2014. Diabetes. 63:4302. PubMed
  4. Zachman A, et al. 2014. Biomaterials. 35:9635. PubMed
  5. Justice J, et al. 2015. J Appl Physiol. 118:163. PubMed
  6. Chan K, et al. 2015. J Biol Chem. 290: 16979 - 16988. PubMed
  7. Schaafsma W, et al. 2015. Brain Behav Immun. 48: 205-221. PubMed
  8. Ye H, et al. 2016. Exp Mol Pathol. 100: 307-311. PubMed
  9. Naji A, et al. 2016. Sci Rep. 6: 26162. PubMed
  10. Lehmann M, et al. 2016. J Leukoc Biol. 99: 1057 - 1064. PubMed
  11. Clausen B, et al. 2016. Sci Rep. 6:29291. PubMed
  12. Shih S, et al. 2016. PLoS One. 11: 0157647. PubMed
  13. Fante T, et al. 2016. PLoS One. 11: 0160184. PubMed
  14. Brunetti J, et al. 2016. J Biol Chem. 291(49):25742-25748. PubMed
  15. Wang C, et al. 2020. Redox Biol. 37:101688. PubMed
  16. Ci T, et al. 2020. Sci Adv. 6:00. PubMed
  17. Al Mamun A, et al. 2020. Proc Natl Acad Sci U S A. 117:1742. PubMed
  18. Nagata K, et al. 2020. Int Immunol. 32:143. PubMed
  19. Schaafsma W, et al. 2017. Neurobiology of Disease. 10.1016/j.nbd.2017.07.017. PubMed
  20. Xiahou Z,et al. 2017. Nat Commun. . 10.1038/s41467-017-00930-9. PubMed
  21. Sun T, et al. 2018. Front Immunol. 9:1245. PubMed
  22. Liu R, et al. 2018. Exp Ther Med. 15:3385. PubMed
  23. Liu TH, et al. 2018. Nutrients. 10:E472. PubMed
  24. Zhou XM, et al. 2018. Front Pharmacol. 8:904. PubMed
  25. Li DY, et al. 2018. Sci Rep. 8:17979. PubMed
  26. Chen Y, et al. 2018. Exp Ther Med. 16:4322. PubMed
  27. Luo W, et al. 2018. Bioorg Med Chem. 26:1547. PubMed
  28. Dai X, et al. 2017. FASEB J. 31:2881. PubMed
  29. Medrano–Jiménez E, et al. 2019. J Neuroinflammation. 16:143. PubMed
  30. Du Q, et al. 2019. Front Microbiol. 10:2050. PubMed
  31. Park GT, et al. 2019. Front Immunol. 10:2095. PubMed
  32. Wilson JL, et al. 2020. Cell Rep. 30:1542. PubMed
  33. Dong X, et al. 2019. ACS Nano. 13:1272. PubMed
  34. Kim MW, et al. 2020. Front Bioeng Biotechnol. 8:133. PubMed
  35. Yang Y, et al. 2020. J Int Med Res. 48:300060520902605. PubMed
  36. Sun B, et al. 2018. Cell Mol Immunol. 1.168055556. PubMed
  37. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  38. Park HB, et al. 2020. Oncoimmunology. 9:1772663. PubMed
  39. Qiu J, et al. 2010. Appl Envir Microbiol. 76:5846. PubMed
  40. Cobb C 2010. J Immunol. 184:3789. PubMed
  41. Chavant F, et al. 2010. J Pharmacol Exp Ther. 332:505. PubMed
  42. Yang H, et al. 2011. J Cell Sci. 124:2552. PubMed
4 pg/mL
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealer
  • Absorbent paper

Antigen Details

Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Type
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Gene ID
21926 View all products for this Gene ID
View information about TNF-alpha on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 4    Revision Date: 12.14.2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587


Login / Register
Remember me
Forgot your password? Reset password?
Create an Account