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MojoSort™ Human CD14 Nanobeads

Pricing & Availability
Regulatory Status
RUO
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Product Citations
publications
Mojosort_Human_CD14_Nanobeads_1_082317
PBMCs were prepared to isolate or deplete CD14+ monocytes using the MojoSort™ Human CD14 Nanobeads. Top plot: CD14+ monocytes after isolation. Bottom plot: Cells before isolation. Cells were stained with CD4 (clone RPA-T4) Brilliant Violet 421™ and CD14 (clone HCD14) PE. Dead cells were excluded by 7-AAD.
  • Mojosort_Human_CD14_Nanobeads_1_082317
    PBMCs were prepared to isolate or deplete CD14+ monocytes using the MojoSort™ Human CD14 Nanobeads. Top plot: CD14+ monocytes after isolation. Bottom plot: Cells before isolation. Cells were stained with CD4 (clone RPA-T4) Brilliant Violet 421™ and CD14 (clone HCD14) PE. Dead cells were excluded by 7-AAD.
  • Mojosort_Human_CD14_Nanobeads_2_082317
Cat # Size Price Quantity Check Availability Save
480093 20 tests 198 CHF
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480094 200 tests 740 CHF
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Description

CD14+ expressing cells are either selected or depleted by incubating your sample with the directly conjugated CD14 Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD14+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.

MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.

Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents

For Cat# 480093:

  • 1 vial containing 100 µl of Human TruStain FcX™ (Fc Receptor Blocking Solution)
  • 1 vial containing 200 µl of Human CD14 Nanobeads

For Cat# 480094:

  • 1 vial containing 1 ml of Human TruStain FcX™ (Fc Receptor Blocking Solution)
  • 2 vials containing 1 ml of Human CD14 Nanobeads

Product Details

Verified Reactivity
Human
Formulation
Aqueous solution containing 0.05% sodium azide and 0.3% BSA.
Preparation
Conjugated nanobeads: Protein-coated magnetic beads.
Storage & Handling
All components should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested
Recommended Usage

5 µl of Human TruStain FcX™ (Fc Receptor Blocking Solution) for 1 x 107 cells in 100 µl of buffer.
10 µl of Human CD14 Nanobeads for 1 x 107 cells in 100 µl of buffer.

Application Notes

The expected purity is >90% CD14+ cells post-separation of human peripheral blood mononuclear cells from healthy donors, analyzed by flow cytometry

This kit is designed for the positive selection or depletion of human CD14+ cells from peripheral blood mononuclear cells (PBMCs).

Antibody or cocktail dilution to use in column: N/A
Nanobead dilution to use in columns: 10X

To find out the dilution to use this product in separation columns please download the CoA.

Product Citations
  1. Kiritsy MC, et al. 2021. Elife. 10:. PubMed
  2. Gopinath A, et al. 2021. NPJ Parkinsons Dis. 7:62. PubMed

Related FAQs

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

What antibodies are present in the depletion cocktails provided for isolation kits?

Please contact our technical service team for further assistance.

Go To Top Version: 2    Revision Date: 07.25.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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