LEGENDplex™ Human VEGF Capture Bead B6, 13X

Pricing & Availability
Regulatory Status
RUO
Other Names
VEGFA, MVCD1, VEGF, Vascular permeability factor (VPF), Vascular Endothelial Growth Factor A121, VEGF-A
Ave. Rating
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Product Citations
publications
1_740710-VEGF-Solo-Curve
Data generated using the following LEGENDplex™ Mix & Match Reagents:

• Human VEGF Capture Bead B6, 13X (Cat. # 740710)
• Human Angiogenesis Panel 1 Detection Antibodies (Cat. # 740700)
• Human Angiogenesis Panel 1 Standard (Cat. # 740699)
• Buffer Set I (Cat. # 740623)

Note: Data acquired on a BD FACSCalibur™ flow cytometer.
  • 1_740710-VEGF-Solo-Curve
    Data generated using the following LEGENDplex™ Mix & Match Reagents:

    • Human VEGF Capture Bead B6, 13X (Cat. # 740710)
    • Human Angiogenesis Panel 1 Detection Antibodies (Cat. # 740700)
    • Human Angiogenesis Panel 1 Standard (Cat. # 740699)
    • Buffer Set I (Cat. # 740623)

    Note: Data acquired on a BD FACSCalibur™ flow cytometer.
  • 2_740710-VEGF-Detectability
    Human plasma samples (n = 80) were quantified using the following LEGENDplex™ Mix & Match Reagents:

    • Human VEGF Capture Bead B6, 13X (Cat. # 740710)
    • Human Angiogenesis Panel 1 Detection Antibodies (Cat. # 740700)
    • Human Angiogenesis Panel 1 Standard (Cat. # 740699)
    • Buffer Set I (Cat. # 740623)

    Data are presented as Mean + SD. Plasma samples were provided courtesy of Professor Huaizhu Wu, Baylor College of Medicine.
Cat # Size Price  
740710 100 tests (270 µL) 100,00 CHF
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To purchase, view our LEGENDplex™ Mix and Match page

Description

The LEGENDplex™ Human Angiogenesis  Panel 1 Mix and Match Capture Beads offer flexible customization within the panel (12-plex) using the following items listed below:

740597 LEGENDplex™ Human IL-6 Capture Bead A4, 13X
740701 LEGENDplex™ Human Angiopoietin-1 Capture Bead A5, 13X
740702 LEGENDplex™ Human Angiopoietin-2 Capture Bead A6, 13X
740703 LEGENDplex™ Human EGF Capture Bead A7, 13X
740704 LEGENDplex™ Human IL-34 Capture Bead A10, 13X
740705 LEGENDplex™ Human FGF-basic Capture Bead A10, 13X
740706 LEGENDplex™ Human CXCL8 (IL-8) Capture Bead B2, 13X
740707 LEGENDplex™ Human PECAM-1 Capture Bead B3, 13X
740708 LEGENDplex™ Human PlGF Capture Bead B4, 13X
740710 LEGENDplex™ Human VEGF Capture Bead B6, 13X
740711 LEGENDplex™ Human TNF-α Capture Bead B7, 13X
740712 LEGENDplex™ Human G-CSF Capture Bead B9, 13X

Selected items will be used together with:

740699 LEGENDplex™ Human Angiogenesis  Panel 1 Standard
740700 LEGENDplex™ Human Angiogenesis  Panel 1 Detection Antibodies
740623 LEGENDplex™ Buffer Set I
740377 LEGENDplex™ Filter Plate or 740379 LEGENDplex™ V-bottom Plate

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Application

Multiplex


Learn more about LEGENDplex™ at biolegend.com/legendplex

Download the LEGENDplex™ software here.

Antigen Details

Biology Area
Cell Biology, Neuroscience, Stem Cells, Synaptic Biology, Angiogenesis
Molecular Family
Growth Factors, Cytokines/Chemokines
Gene ID
7422 View all products for this Gene ID
UniProt
View information about VEGF on UniProt.org

Related FAQs

If I don't have a vacuum, how do I remove the liquid from my plate?

If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.

Should I perform the assay with the filter plates or with V-bottom plates?

Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.

After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?

The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.

What is the shelf life of LEGENDplex™ kits?

LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.

Is special software required for data analysis?

Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.

Go To Top Version: 1    Revision Date: 03.01.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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