- Other Names
- MRP8/MRP14 heterodimer, Myeloid-related protein 8/14, S100A8/A9, Calprotectin
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|740556||100 tests (270 µL)||100 CHF|
The Vascular Inflammation Panel 1 Mix and Match Capture Beads offer flexible customization within the panel (13-plex) with the following items listed below:
740555 LEGENDplex™ Human Myoglobin Capture Bead A4, 13X
740556 LEGENDplex™ Human MRP8/14 Capture Bead A5, 13X
740557 LEGENDplex™ Human NGAL Capture Bead A6, 13X
740558 LEGENDplex™ Human CRP Capture Bead A7, 13X
740559 LEGENDplex™ Human MMP-2 Capture Bead A8, 13X
740560 LEGENDplex™ Human OPN Capture Bead A10, 13X
740561 LEGENDplex™ Human MPO Capture Bead B2, 13X
740562 LEGENDplex™ Human SAA Capture Bead B3, 13X
740476 LEGENDplex™ Human IGFBP-4 Capture Bead B4, 13X
740563 LEGENDplex™ Human ICAM-1 Capture Bead B5, 13X
740564 LEGENDplex™ Human VCAM-1 Capture Bead B6, 13X
740565 LEGENDplex™ Human MMP-9 Capture Bead B7, 13X
740566 LEGENDplex™ Human Cystatin C Capture Bead B9, 13X
Selected items will be used together with:
740554 LEGENDplex™ Human Vascular Inflammation Panel 1 Standard
740553 LEGENDplex™ Human Vascular Inflammation Panel 1 Detection Antibodies
740374 LEGENDplex™ Buffer Set C
740377 or 740379 Filter Plate or V-bottom Plate
The assembled Human Vascular Inflammation Mix and Match Panel 1 is a multiplex bead-based assay kit, using fluorescence–encoded beads suitable for use on various flow cytometers. This allows simultaneous quantification of up to 13 human proteins: Myoglobin, MRP8/14, NGAL, CRP, MMP-2, OPN, MPO, SAA, IGFBP-4, ICAM-1, VCAM-1, MMP-9, and Cystatin C. This assay panel provides higher detection sensitivities and broader dynamic ranges than traditional ELISA methods. The panel has been validated for use with serum, plasma, tissue culture supernatant, and urine samples.Product Details
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- If I don't have a vacuum, how do I remove the liquid from my plate?
Do NOT invert the plate to dump the liquid. The beads will not adhere to the plate. After centrifugation using a swing bucket rotor with a plate adaptor, you can remove the liquid by using a pipette.
- Is special software required for data analysis?
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). FCS files can be analyzed by different software. Data generated from using LEGENDplex™ kits can be analyzed using the LEGENDplex™ data analysis software freely available on the BioLegend website (biolegend.com/legendplex/software). A free of charge software license dongle is currently provided in the kit. Please check our website for the most updated versions of the software.
- Should I perform the assay with the filter plates or with V-bottom plates?
Filter plates or V-bottom plates have been included in some kits for your convenience. Performing the assay with filter plates is generally recommended. A vacuum filtration unit is required to work with the filter plates. If however, you don’t have access to a vacuum manifold, then you can use the V-bottom plates or micro-FACS tubes as described in the manual and follow the recommended assay protocols for the type of plates or tubes you choose. All tubes and plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- What is the shelf life of LEGENDplex™ kits?
LEGENDplex™ kits are valid for 6 months from the date of receipt.