LEGEND MAX™ Mouse IL-23 (p19/p40) ELISA Kit

Pricing & Availability
Other Names
IL-23 (p19/p40) Pre-coated ELISA Kit,
Ave. Rating
3 reviews
Product Citations
publications
IL-23 061709
  • IL-23 061709
Cat # Size Price Quantity Save
433707 1 Pre-coated Plate 295 CHF
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Description

Mouse IL-23 (Interleukin 23 p19/p40, P19, IL-23A, IL23p19) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. IL-23 is secreted by activated macrophages and dendritic cells. Human and mouse p19 share 70% amino acid sequence identity. IL-23 has been shown to enhance IFN-? production by memory T cells. Additionally, mouse IL-23 can activate mouse memory T cells to produce the potent proinflammatory cytokine IL-17. IL-23 is upregulated in certain autoimmune diseases and promote immunity in response to some viral and mycobacterial infections. The protein is implicated in inflammatory diseases such as rheumatoid arthritis and Crohn’s disease.

BioLegend’s LEGEND MAX™ Mouse IL-23 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Mouse IL-23 specific monoclonal antibody is pre-coated on a 96-well strip-well plate.

BioLegend’s LEGEND MAX™ Mouse IL-23 ELISA Kit is specifically designed for the accurate quantitation of Mouse IL-23 from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Anti-Mouse IL-23 Pre-coated 96-well Strip Microplate
  • Mouse IL-23 Dectection Antibody
  • Mouse IL-23 Standard
  • Matrix A (for serum and plasma samples only)
  • Avidin-HRP A
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution E
  • Stop Solution
  • Plate Sealers

Product Details

Reactivity
Mouse
Application

ELISA

Application References

(PubMed link indicates BioLegend citation)
  1. Riol-Blanco L, et al. 2010. J. Immunol. 184:1710. PubMed
  2. Zhang Y, et al. 2010. J. Immunol. 184:3866. PubMed
Product Citations
  1. Ma Y 2010. J Immunol. 184:3866. PubMed
  2. Riol-Blanco L, et al. 2010. J Immunol. 184:1710. PubMed
Publication Library
Sensitivity
4.8 pg/mL
Standard Range
31.3-2,000 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
Dendritic cells, monocytes/macrophages, B cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Gene ID
16160 View all products for this Gene ID 83430 View all products for this Gene ID
UniProt
View information about IL-23 p19/p40 on UniProt.org

Related FAQs

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

Dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The Wash buffer is the same for all the current LEGEND MAX™ kits. All the part numbers on the Wash Buffer bottles in these kits should be the same. For ELISA MAX™ Deluxe and ELISA MAX™ Standard sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

In your LEGEND MAX™ ELISA Kits, there is a step that calls for a washing of the plates before even adding any sample to it. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The washings were designed to remove these components before you start the assay. If you do not do the washings, the effect on assay performance is negligible.

Go To Top Version: 2    Revision Date: 09.11.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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