Pricing & Availability
Regulatory Status
Other Names
MIF Pre-coated ELISA,
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Product Citations
  • MIF_Human_110911
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438407 1 Pre-coated Plate 462 CHF
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Human Macrophage Migration Inhibitory Factor (MIF) is a 12.5 kD, 115 amino acid, non-glycosylated polypeptide and is expressed by multiple cell types in­cluding activated T cells, macrophages, eosinophils, epithelial cells, and endo­thelial cells. MIF plays many roles in biological processes such as catalytic activity, immu­nity, endocrine regulation, signal modulation, and inflammation. MIF is expressed in malignant cells including lung, liver, breast, colon, and prostate tumors.  Recombinant MIF is a mixture of monomers, dimers, and trimers. The physiologically active forms are believed to be predominantly the dimeric and trimeric forms1, 2.

BioLegend’s LEGEND MAX™ Human Active MIF ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with 96 well microtiter strip plates that are pre-coated with a capture antibody, specific for dimeric, trimeric or higher levels of MIF complex.

This kit is specifically designed for the accurate quantitation of human active MIF from cell culture supernatant, serum, plasma and other biological fluids. This kit is analytically validated, with ready-to-use reagents.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Anti-Human MIF Pre-coated 96-well Strip Microplate
  • Human MIF Dectection Antibody
  • Human MIF Standard
  • Avidin-HRP A
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity


Product Citations
  1. Abdul-Aziz A, et al. 2017. Cancer Res . 77(2):303-311. PubMed
17.4 ± 9.2 pg/mL
Standard Range
125-8,000 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
Activated T cells, macrophages, eosinophils, epithelial cells, and endothelial cells
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Antigen References

1. Potolicchio I, et al. 2003. J. Biol. Chem. 278:30889.
2. Meyer-Siegler K, et al. 2005. BMC Cancer 5:73.

Gene ID
4282 View all products for this Gene ID
View information about MIF on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 2    Revision Date: 09.11.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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