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LEAF™ Purified anti-human IL-21 Antibody

Pricing & Availability
Clone
Poly5343 (See other available formats)
Regulatory Status
RUO
Other Names
B cell growth factor 1 (Interleukin 21)
Isotype
Goat Polyclonal IgG
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Product Citations
publications
Poly5343_LEAF_IL-21_Antibody_1_020821
Recombinant human IL-21 (Cat. No. 571204) (black circles) stimulates the production of human IFN-γ in human NK-92 cells. LEAF™ purified anti-human IL-21 antibody (Poly5343) (purple squares) inhibits the effect of recombinant human IL-21 at 5 ng/mL on human NK-92 cells in a dose-dependent manner whereas the LEAF™ purified goat IgG isotype ctrl antibody (gray triangles) does not have the effect. ND50 range: 0.4 - 2 µg/mL.
  • Poly5343_LEAF_IL-21_Antibody_1_020821
    Recombinant human IL-21 (Cat. No. 571204) (black circles) stimulates the production of human IFN-γ in human NK-92 cells. LEAF™ purified anti-human IL-21 antibody (Poly5343) (purple squares) inhibits the effect of recombinant human IL-21 at 5 ng/mL on human NK-92 cells in a dose-dependent manner whereas the LEAF™ purified goat IgG isotype ctrl antibody (gray triangles) does not have the effect. ND50 range: 0.4 - 2 µg/mL.
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534303 100 µg 440 CHF
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Description

IL-21 was identified by a functional screening from conditioned medium of activated T cells using Baf3-IL-21R transfectants. In normal B cells, IL-21 can mediate cell proliferation, growth arrest, and terminal differentiation, or apoptosis, depending on their activation status. IL-21 enhances B cell proliferation following incubation with an activating CD40 antibody. This result suggests that IL-21 enhances B cell function following T and B cell interactions. Nevertheless, IL-21 inhibits the proliferation of murine and human B cells stimulated with anti-IgM antibodies. In addition, IL-21 enhances proliferation, cytotoxic activity, and IFN-γ production by CD8-effector T cells, and induces terminal differentiation of activated natural killer (NK) cells. Also, IL-21 can drive Th17 responses in conjunction with TGF-β. Nevertheless, IL-21 is not essential for the differentiation of Th17 cells in vitro or in vivo, as it was showed using IL-21 and IL-21R-deficient mice. IL-6 induces the production of IL-21 from CD4 T cells upon TCR stimulation. In addition, IL-6 and IL-21 are key players in the Tfh differentiation, characterized by increased protein expression of both Bcl-6 and CXCR5. IL-21R complex is formed with the IL-21R-alpha chain and the common subunits (γc) shared with other interleukins, such as IL-2, IL-4, IL-7, IL-9, and IL-15.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant human IL-21
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by the neutralization of human IFN-γ production induced by recombinant human IL-21 (5 ng/mL) (Cat. No. 571204) on human NK-92 cells. ND50 range: 0.4 - 2 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application

RRID
AB_2894672 (BioLegend Cat. No. 534303)

Antigen Details

Structure
Interleukin
Distribution

CD4 activated T cells, Th17 cells, NK T cells

Function
IL-21 induces T and B cell proliferation, B cell Ig class switching to IgG production, decreases dendritic cell function, enhances differentiation of effector and central memory T cells, and regulates T cell and hematopoietic progenitor cell homeostasis.
Interaction
T cells, B cells, NK cells, dendritic cells, and some nonimmune cells, such as fibroblasts and epithelial cells
Ligand/Receptor
IL-21R and the common γc subunit (CD132) are components of the IL-21R complex.
Cell Type
B cells, Dendritic cells, Hematopoietic stem and progenitors, NKT cells, T cells, Th17, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Parrish-Novak J, et al. 2000. Nature. 408:57-63.
  2. de Totero D, et al. 2006. Blood. 107:3708-15.
  3. Korn T, et al. 2007. Nature. 448:484-487.
  4. Coquet JM, et al. 2008. J Immunol. 180:7097-101.
  5. Dienz O, et al. 2009. J Exp Med. 206:69-78.
  6. Kaplan MH, et al. 2011. Blood. 117:6198-201.
  7. Eto D, et al. 2011. PLoS One. 6:e17739.
Gene ID
59067 View all products for this Gene ID
UniProt
View information about IL-21 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 02.08.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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