LEAF™ Purified anti-human IL-7 Antibody

Pricing & Availability
Clone
BVD10-40F6 (See other available formats)
Other Names
Interleukin-7, Lymphopoietin-1 (LP-1), Pre-B cell growth factor, Thymocyte growth factor
Isotype
Rat IgG1, κ
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Cat # Size Price Quantity Avail. Save
501303 50 µg 90 CHF
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501304 500 µg 280 CHF
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Description

IL-7 is a potent lymphoid cell growth factor which promotes the proliferation of pre-B cells, thymocytes, T cell progenitors, and mature T cells. IL-7 induces formation of LAK cells and CTLs, and can stimulate tumoricidal activity of monocytes and macrophages. IL-7 can induce upregulation of proinflammatory cytokines in myeloid cells. IL-7 is expressed by stromal cells. The BVD10-40F6 antibody can neutralize the bioactivity of natural or recombinant IL-7.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli - expressed, recombinant human IL-7
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

ELISA Capture, ELISPOT Capture, Neut, WB

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA or ELISPOT capture applications, a concentration range of 2-6 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-7 recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture1,2: The Purified BVD10-40F6 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated BVD10-11C10 antibody (Cat. No. 506602) as the detecting antibody. The LEAF™ Purified antibody is suggested for ELISPOT capture.
Neutralization1: The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human IL-7 bioactivity (Cat. No. 501304).
Additional reported applications (for the relevant formats) include: Western blotting.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5.
  2. Abrams J. 1995. Curr. Prot. Immunol.. John Wiley and Sons New York. Unit 6.20.
RRID
AB_315165 (BioLegend Cat. No. 501303)
AB_315166 (BioLegend Cat. No. 501304)

Antigen Details

Structure
Cytokine; 20-28 kD (Mammalian)
Bioactivity
Proliferation of pre-/pro-B cells, thymocytes, T cells; formation of LAK cells, CTLs, stimulation of tumoricidal activity monocytes/macrophages; upregulate proinflammatory cytokines in myeloid cells
Cell Sources
Adherent bone marrow stromal cells, thymic stromal cells, spleen, keratinocytes
Cell Targets
Progenitor B and T cells; CD4-CD8- thymocytes; megakaryocytes
Receptors
Heterodimer IL-7Rα (CD127); γ-subunit (CD132) in common with IL-2R, IL-4R, IL-13R, IL-15R
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Park L, et al. 1992. Adv. Exp. Med. Biol. 323 125.
3. Melchers F, et al. 1992. Adv. Exp. Med. Biol. 323 111.

Regulation
Downregulated by TGF-β
Gene ID
3574 View all products for this Gene ID
UniProt
View information about IL-7 on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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