Go-ChIP-Grade™ Purified Mouse IgG2a, κ isotype Ctrl Antibody

Pricing & Availability
Clone
MOPC-173 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
MOPC-173_GoChIP_IgG2a_Antibody_060618_updated
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 x 106 HeLa cells with either A)1:300 dilution of Go-ChIP-Grade™ Purified anti-RNA Polymerase II RPB1 antibody (clone 8WG16, Cat. No. 664911) or B) equal amount of Go-ChIP-Grade™ purified mouse IgG2a, κ isotype control antibody (clone MOPC-173, Cat. No. 400287) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human GAPDH gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • MOPC-173_GoChIP_IgG2a_Antibody_060618_updated
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 x 106 HeLa cells with either A)1:300 dilution of Go-ChIP-Grade™ Purified anti-RNA Polymerase II RPB1 antibody (clone 8WG16, Cat. No. 664911) or B) equal amount of Go-ChIP-Grade™ purified mouse IgG2a, κ isotype control antibody (clone MOPC-173, Cat. No. 400287) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human GAPDH gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
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400287 25 µg 20,00 CHF
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400288 100 µg 80,00 CHF
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This product is scheduled for discontinuation. Please use the availability checker to determine current availability. Cat. Nos. 400201 and 400202 are available as an alternative.
Description

The MOPC-173 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat, and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
FC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by ChIP assay as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.

Application Notes

Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA).

Related FAQs

IgG2a gene is deleted in some mouse strains, which ones are they?
IgG2a gene is deleted in C57Bl/6, C57Bl/ 10, SJL, and NOD mice. It is replaced with IgG2c gene instead.
Go To Top Version: 1    Revision Date: 05.23.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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