- Clone
- A17016B (See other available formats)
- Other Names
- Signal transducer and activator of transcription 5A
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 x 106 of Ba/F3 cells deprived for IL-3 overnight and then treated with IL-3 with either A)1:50 dilution of Go-ChIP-Grade™ Purified anti-STAT5 Phospho (Tyr694) (Clone A17016B) or B) equal amount of Purified Mouse IgG1, κ Isotype Control Antibody (Clone MG1-45, Cat. No. 401401) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting mouse SOCS3 gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin. -
Human peripheral blood mononuclear cells were treated with (filled histogram), or without (open histogram) Recombinant Human IL-2 (carrier free, Cat. No. 589102) for 15 minutes, fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401) then stained with Go-ChIP-Grade™ Purified anti-STAT5 Phospho (Tyr694) (clone A17016B), and followed by mouse IgG PE. Data was gated on the lymphocyte population.
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 699603 | 25 µg | 95 CHF | ||||
| 699604 | 100 µg | 225 CHF | ||||
STAT5 is a member of the signal transducer and activator of transcription factors (STAT) family, mediating growth and cytokine signaling. STAT5 consists of two closely related family members, STAT5A and STAT5B, which exhibit 96% sequence homology and are functionally redundant. Upon activation, STAT5 is phosphorylated by receptor tyrosine kinases and, in turn, forms homodimers or hererodimers with other family members through its SH2 domains. The dimerized STAT5 translocates to the nucleus and binds to the STAT5 response element (TTCXXXGAA). STAT5 plays crucial regulatory roles in cell proliferation, anti-apoptosis, early B cell differentiation, and T cell development.
Product DetailsProduct Details
- Reactivity
- Human, Mouse†
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Phosphorylated human STAT5A at Tyr694
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ChIP - Quality tested
ICFC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50 - 1:200 by volume. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent to be titrated for optimal performance before each experiment.
- Application Notes
-
Does not cross-react with mouse for ICFC.
†Based on in-house testing, clone A17016B cross-reacts to Mouse STAT5 Phospho (Tyr694) in Western blots, but it is not recommended for intracellular flow cytometry application due to dim staining - RRID
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AB_2734545 (BioLegend Cat. No. 699603)
AB_2734546 (BioLegend Cat. No. 699604)
Antigen Details
- Structure
- 794 amino acids, predicted molecular weight of 91 kD; contains a SH2 domains responsible for homodimerization or heterodimerization.
- Distribution
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Cytoplasm; phosphorylated form translocates to the nucleus.
- Function
- STAT5 is a transcription regulator and is involved in cell differentiation, proliferation, and survival, in response to several cytokines, such as interleukins, erythropoietin, growth hormone, thrombopoietin, and prolactin.
- Interaction
- Forms a homodimer or a heterodimer with other family members. Interacts with NCOA1, SOCS7, CBP, p300, SMRT and ERBB4.
- Cell Type
- B cells, Th17, Tregs
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction, Transcription Factors
- Molecular Family
- Nuclear Markers, Phospho-Proteins
- Antigen References
-
1. Bell BD, et al. 2013. Nat. Immunol. 14:364.
2. Cholez E, et al. 2012. Leukemia. 26:2390.
3. Heltemes-Harris LM, et al. 2012. Curr. Opin. Immunol. 24:146.
4. Heltemes-Harris LM, et al. 2011. Ann. N Y Acad. Sci. 1217:18.
5. Malin S, et al. 2010. Curr. Opin. Immunol. 22:168.
6. Xu J, et al. 2009. J. Neurosci. 29:2022. - Gene ID
- 6776 View all products for this Gene ID
- UniProt
- View information about STAT5 Phospho on UniProt.org
Related Pages & Pathways
Pathways
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