Go-ChIP-Grade™ Purified anti-STAT3 Phospho (Ser727) Antibody

Pricing & Availability
Clone
A16089B (See other available formats)
Other Names
Signal transducer and activator of transcription 3, Acute-phase response factor (APRF), HIES, ADMIO
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A16089B_GoChIP_STAT3_Phospho_Antibody_071717
Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3 µg of cross-linked chromatin samples from HeLa cells starved overnight and then treated with IL-6 with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-STAT3 Phospho (Ser727) Antibody (Clone A16089B), B) equal amount of Purified Mouse IgG1, κ Isotype Control Antibody (#400101), or C) competitor’s ChIP-grade Purified anti-STAT3 Phospho (Ser727) Antibody and D) equal amount of matched Isotype Control Antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human IRF1 gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • A16089B_GoChIP_STAT3_Phospho_Antibody_071717
    Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3 µg of cross-linked chromatin samples from HeLa cells starved overnight and then treated with IL-6 with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-STAT3 Phospho (Ser727) Antibody (Clone A16089B), B) equal amount of Purified Mouse IgG1, κ Isotype Control Antibody (#400101), or C) competitor’s ChIP-grade Purified anti-STAT3 Phospho (Ser727) Antibody and D) equal amount of matched Isotype Control Antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human IRF1 gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • A16089B_GoChIP_STAT3_Phospho_Antibody_WB_071717
    Total cell lysate from Jurkat cells serum starved overnight (lane 1, 3, 5; 5 µg), or serum starved overnight and then treated with PMA (lane 2, 4, 6; 5 µg) were resolved by electrophoresis (4-12% Bis-Tris), transferred to nitrocellulose, and probed with 2µg/ml (lane 1, 2), 1µg/ml (lane 3, 4) or 0.5µg/ml (lane 5, 6) Go-ChIP-Grade7trade; Purified anti-STAT3 Phospho (Ser727) (clone A16089B) antibody. Proteins were visualized using an HRP Goat anti-mouse IgG (clone Poly40532) antibody and chemiluminescence detection. Anti-STAT3 (clone 4G4B45) antibody was used as a loading control.
Cat # Size Price Quantity Avail. Save
698903 25 µg 75 CHF
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698904 100 µg 235 CHF
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Description

The STAT3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors and is required for murine fetal development. STAT3 is an 88 kD member of the STAT (signal transducer and activators of transcription) protein family that is phosphorylated in response to a cytokine receptor-associated kinase activity. Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding. Transcriptional activation was reported to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways.

STAT3 forms both homo- and heterotrimers and is involved in the activation of genes required for cell growth and apoptosis. STAT3 is also involved in gp130 signaling and binds to IL-6 response elements in various acute phase protein promoters. STAT3 is phosphorylated by signaling from IFNs, EGF, FGF, IL-5, HGF, LIF, and BMP2. STAT3 activity is inhibited by PIAS3 and GRIM-19 and can also be regulated by the Rac1 protein.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Stat3 peptide phosphorylated at Ser 727. Complete Freund's adjuvant.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
WB - Verified 

Recommended Usage

Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50-1:100 by volume. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The STAT3 Phospho (Ser727) antibody recognizes the regulatory serine phosphorylation of human STAT3 protein.

RRID
AB_2715807 (BioLegend Cat. No. 698903)
AB_2715808 (BioLegend Cat. No. 698904)

Antigen Details

Structure
STAT3 is a 770 amino acid protein of 88 kD. It consists of a DNA binding domain, a SH2 domain, a regulatory tyrosine responsible for binding of SH2 domain, and a C-terminal transactivation domain.
Distribution

Ubiquitous

Function
STAT3 is also involved in gp130 signaling and binds to IL-6 response elements in various acute phase protein promoters. STAT3 is phosphorylated by signaling from IFNs, EGF, FGF, IL-5, HGF, LIF, and BMP2. STAT3 activity is inhibited by PIAS3 and GRIM-19 and can also be regulated by the Rac1 protein.
Interaction
When activated, STAT3 dimerizes and translocates to the nucleus where it regulates gene expression.
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Transcription Factors
Molecular Family
Phospho-Proteins, Nuclear Markers
Antigen References

1. Akira S, et al. 1994. Cell. 77:63.
2. Zhang X, et al. 1995. Science 267:1990.
3. Sanchez-Margalet V, et al. 2001. Cell. Immunol. 211:30.
4. Simon A, et al. 2000. Science 290:144.
5. Hoey T, et al. 1999. Adv. Immunol. 71:145.

Gene ID
6774 View all products for this Gene ID
UniProt
View information about STAT3 Phospho (Ser727) on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 07.20.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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