Go-ChIP-Grade™ Purified anti-STAT3 Antibody

Pricing & Availability
Clone
P83B5 (See other available formats)
Regulatory Status
RUO
Other Names
Signal transducer and activator of transcription 3, Acute-phase response factor (APRF), HIES, ADMIO
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P83B5_GoChIP_STAT3_Antibody_091117
Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3 µg of cross-linked chromatin samples from HeLa cells starved overnight and then treated with IL-6 with either A) 1:50 dilution of Go-ChIP-Grade™ purified anti-STAT3 (clone P83B5), or B) equal amount of purified Mouse IgG1, κ isotype control antibody. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human IRF1 gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • P83B5_GoChIP_STAT3_Antibody_091117
    Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3 µg of cross-linked chromatin samples from HeLa cells starved overnight and then treated with IL-6 with either A) 1:50 dilution of Go-ChIP-Grade™ purified anti-STAT3 (clone P83B5), or B) equal amount of purified Mouse IgG1, κ isotype control antibody. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human IRF1 gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • P83B5_PURE_STAT3_Antibody_093016
    Total cell lysates (15 µg protein) from Raw264.7 (lane 1), PC3 (lane 2) and HeLa (lane 3) and Molt-4 (lane 4) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1 µg/mL (1:500 dilution) of Purified anti-STAT3 Antibody (clone P83B5) (upper panel). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for anti-STAT3 Antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-Actin antibody, clone 2F1-1(lower panel) as a loading control. Lane M: Molecular weight ladder.
Cat # Size Price Quantity Check Availability Save
691503 25 µg 75,00 CHF
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691504 100 µg 235,00 CHF
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This product is scheduled for discontinuation. Please use the availability checker to determine current availability. Cat. No. 691502 is available as an alternative.
Description

STAT3 is an 88 kD member of the STAT (signal transducer and activators of transcription) protein family that is phosphorylated in response to a cytokine receptor-associated kinase activity. Phosphorylation of STAT3 induces nuclear translocation to activate transcription. STAT3 forms both homo- and heterotrimers and is involved in the activation of genes required for cell growth and apoptosis. STAT3 is also involved in gp130 signaling and binds to IL-6 response elements in various acute phase protein promoters. STAT3 is phosphorylated by signaling from IFNs, EGF, FGF, IL-5, HGF, LIF, and BMP2. STAT3 activity is inhibited by PIAS3 and GRIM-19 and can also be regulated by the Rac1 protein.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant fragment (aa 20-240) of human STAT3 expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.05% sodium azide.
Preparation
The Go-ChIP-Grade™ Purified antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
WB - Verified

Recommended Usage

Each lot of this antibody is quality control tested by ChIP. For ChIP, the suggested use of this reagent is 1-50-1:150 dilution by volume. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The canonical sequence of STAT3 contains 770 amino acids with a predicted molecular weight of 88 kD. A shorter isoform of STAT3 with 722 amino acids (83 kD) was reported. Clone P83B5 may recognize both isoforms.

25 µg, 100 µg of Go-ChIP-Grade Purified™ antibody can be used for 2-8, 11-33 immunoprecipitations, respectively, at the recommended dilution.

Application References

(PubMed link indicates BioLegend citation)
  1. Akira S, et al. 1994. Cell. 77:63.
  2. Zhang X, et al. 1995. Science 267:1990.
  3. Sanchez-Margalet V & Martin-Romero C. 2001. Cell Immunol. 211:30.
  4. Simon A, et al. 2000. Science 290:144.
  5. Hoey T & Grusby MJ. 1999. Adv. Immunol. 71:145.
RRID
AB_2716049 (BioLegend Cat. No. 691503)
AB_2716050 (BioLegend Cat. No. 691504)

Antigen Details

Structure
STAT3 is a 770 amino acid protein of 88 kD. It consists of a DNA binding domain, a SH2 domain, a regulatory tyrosine responsible for binding of SH2 domain, and a C-terminal transactivation domain.
Distribution

Ubiquitous

Function
Phosphorylated in response to cytokine signaling by receptor-associated kinases, translocates to the nucleus to act as a transcriptional activator, activates a wide variety of genes involved in cell growth and apoptosis, and is involved in gp130-related signaling.
Interaction
STAT3 forms a homodimer or a heterodimer with other STAT family members and interacts with IL31RA, NCOA1, PELP1, SIPAR, SOCS7, STATIP1, CAV2, DAPK3, EIF2AK2, PIAS3, and TMF1.
Cell Type
Embryonic Stem Cells, Mesenchymal Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Akira S, et al. 1994. Cell. 77:63.
2. Zhang X, et al. 1995. Science 267:1990.
3. Sanchez-Margalet V & Martin-Romero C. 2001. Cell Immunol. 211:30.
4. Simon A, et al. 2000. Science 290:144.
5. Hoey T & Grusby MJ. 1999. Adv. Immunol. 71:145.

Gene ID
6774 View all products for this Gene ID
UniProt
View information about STAT3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 09.11.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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