Go-ChIP-Grade™ Purified anti-mouse/human Bcl-6 Antibody

Pricing & Availability
Clone
IG191E/A8 (See other available formats)
Other Names
B-cell lymphoma 6, BCL-5, LAZ3, BCL6A, ZNF51, ZBTB27
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
IG191EslashA8_GoChIP_Bcl6_1_Antibody_052118
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 Raji cells with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-Bcl6 Antibody (clone IG191E/A8, Cat. No. 648309) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG1, κ isotype control antibody (clone MG1-45, Cat. No. 401409) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human HSP90AB gene region or α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • IG191EslashA8_GoChIP_Bcl6_1_Antibody_052118
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 Raji cells with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-Bcl6 Antibody (clone IG191E/A8, Cat. No. 648309) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG1, κ isotype control antibody (clone MG1-45, Cat. No. 401409) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human HSP90AB gene region or α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • IG191EslashA8_PURE_Bcl-6_Antibody_WB_011618
    Total lysates (15 µg protein) from Raji (lane1), HeLa (lane 2) and A20 (lane 3) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) Purified anti-Bcl6 Antibody, clone IG191E/A8 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted anti-Mouse-IgG secondary antibody conjugated to HRP for the anti-Bcl6 Antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-Actin Antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder.
  • IG191EslashA8_PURE_Bcl-6_Antibody_WB_120517
    Total cell lysates (15 µg protein) from Raji (lane 1) and HeLa cells (lane 2) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to a nitrocellulose membrane, and probed with a 1:500 dilution (1 µg/mL) of purified anti-mouse/human Bcl-6 antibody (clone IG191E/A8). Proteins were visualized using chemiluminescence detection after incubation with a 1:3000 dilution of HRP conjugated anti-Mouse-IgG secondary antibody for the anti-mouse/human Bcl-6 Antibody (upper panel) or a 1:5000 dilution of Direct-Blot™ HRP anti-β-Actin (clone 2F1-1, lower panel). Lane M: Molecular weight ladder. Non-specific bands of higher molecular weight than expected may be observed. Titration of the antibody is recommended.
Cat # Size Price Quantity Avail. Save
648309 25 µg 75 CHF
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648310 100 µg 235 CHF
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This product is scheduled for discontinuation. Please use the availability checker to determine current availability. Cat. No. 648301 & 648302 is available as an alternative.
Description

B-cell lymphoma 6 (Bcl-6), is an 80 kD homodimer and member of the BTB-POZ zinc finger family.  It consists of one BTB (POZ) domain and six C2H2-type zinc fingers. Bcl-6 is a transcriptional repressor as well as a master regulator of germinal center reaction. On B cells, Bcl-6 induces proliferation, antibody class switching and affinity maturation, while inhibits its differentiation to plasma cells. On T cells, Bcl-6 induces its differentiation to TFH. This molecule is also expressed in some B cell lymphomas and breast cancer cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Plasmid vector containing cDNA of the Bcl-6 gene
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
WB - Verified

Recommended Usage

Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50-1:100 by volume. For Western blotting, the suggested use of this reagent is 1.0 µg per ml (1:500). It is recommended that the reagent to be titrated for optimal performance before each experiment.

25 µg, 100 µg of Go-ChIP-Grade™ Purified Antibody can be used for 2-4, 11-16 immunoprecipitations, respectively, at the recommended dilution.

Application Notes

This clone is also known as GI191E/A8. Additional reported applications (for the relevant formats) include: immunohistochemical staining of formalin fixed, paraffin embedded tissue sections1, Western blotting1, and immunoprecipitation1.

Application References

(PubMed link indicates BioLegend citation)
  1. Garcfa JF, et al. 2006. J. Histochem Cytochem. 54:31.
RRID
AB_2721593 (BioLegend Cat. No. 648309)
AB_2721594 (BioLegend Cat. No. 648310)

Antigen Details

Structure
Member of the BTB-POZ zinc finger family, 80 kD, homodimer, 1 BTB (POZ) domain, 6 C2H2-type zinc fingers
Distribution

Germinal center B cells, follicular helper T cells (TFH), B cell lymphomas, breast cancer cells

Function
Transcription factor, master regulator of germinal center reaction
Interaction
BLIMP-1
Cell Type
B cells, Tfh
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Ye BH, et al. 1993. Science 262:747.
2. Baron BW, et al. 1993. P. Natl. Acad. Sci. USA 90:5262.
3. Onizuka T, et al. 1995. Blood 86:28.
4. Johnston RJ, et al. 2009. Science 325:1006.
5. Kitano M, et al. 2011. Immunity 34:961.
6. Baumjohann D, et al. 2011. J. Immunol. 187:2089.
7. Tran TH, et al. 2010. Cancer Res. 70:1711.

Gene ID
604 View all products for this Gene ID 12053 View all products for this Gene ID
UniProt
View information about Bcl-6 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 01.16.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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