Go-ChIP-Grade™ Purified anti-Histone H4 Trimethyl (Lys 20) Antibody

Pricing & Availability
Clone
6F8-D9 (See other available formats)
Other Names
Histone H4, histone 1, H4a, H4 histone family, member A, H4K20me3
Isotype
Mouse IgG1
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Product Citations
publications
1_6F8-D9_GoChIP_HistoneH4_Trimethyl_Lys20_Antibody_1_ChIP_050718
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of HeLa cells with either A)1:50 dilution of Go-ChIP-Grade™ Purified anti-Histone H4 Trimethyl (Lys20) Antibody (clone 6F8-D9, Cat. No. 827703) or B) equal amount of Purified Mouse IgG1 Isotype Control Antibody (Clone MG1-45, Cat. No. 401401) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human MYOD1 gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • 1_6F8-D9_GoChIP_HistoneH4_Trimethyl_Lys20_Antibody_1_ChIP_050718
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of HeLa cells with either A)1:50 dilution of Go-ChIP-Grade™ Purified anti-Histone H4 Trimethyl (Lys20) Antibody (clone 6F8-D9, Cat. No. 827703) or B) equal amount of Purified Mouse IgG1 Isotype Control Antibody (Clone MG1-45, Cat. No. 401401) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human MYOD1 gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • 2_6F8-D9_PURE_Histone_H4_Trimethyl_Antibody_WB_120517
    Total lysates (15 µg protein) from Jurkat (lane 1), Raw 264.7 (lane 2) and UMR106 cells (lane 3) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL of purified anti-Histone H4 Trimethyl (Lys20) antibody (clone 6F8-D9). Proteins were visualized using chemiluminescence detection after incubation with a 1:3000 dilution of HRP conjugated anti-Mouse-IgG secondary antibody for anti-Histone H4 Trimethyl (Lys20) (upper panel) or a 1:5000 dilution of Direct-Blot™ HRP anti-β-Actin (clone 2F1-1, lower panel). Lane M: Molecular weight ladder.
  • 3_6F8-D9_PURE_HistoneH4_Trimethyl_Lys20_Antibody_3_IHCP_050718
    IHC staining of anti-Histone H4 Trimethyl (Lys 20, clone 6F8-D9) on formalin-fixed paraffin-embedded rat brain tissue at 10 µg/ml.
Cat # Size Price Quantity Avail. Save
827703 25 µg 75 CHF
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827704 100 µg 235 CHF
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Description

Histone proteins are classified into core histones (H2A, H2B, H3, H4) and linker histones (H1, H5). Core histones form an octamer, which contains two H2A-H2B dimers and one H3-H4 tetramer. Core histones are predominantly globular except for the unstructured N-terminal tails. Posttranslational modifications, such as acetylation, methylation, phosphorylation, ubiquitination, SUMOylation and ADP-ribosylation occur in histone tails.

Histone modifications induce changes of chromatin structure and thereby affect the accessibility of transcription factors, nuclear proteins and enzymes to genomic DNA, resulting in gene activation or repression. It is known that histone modifications play critical roles in DNA repair, DNA replication, transcription regulation, alternative splicing and chromosome condensation and some diseases including autoimmune diseases and cancers.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a synthetic peptide conjugated to KLH containing trimethylated lysine 20 of human Histone H4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
IHC-P, WB - Verified 

Recommended Usage

Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50-1:100 by volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested dilution is 1:100 - 1:500. For Western blotting, the suggested use of this reagent is 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunohistochemistry (IHC) and Western blotting (WB).

Additional Product Notes

25 µg and 100 µg of Go-ChIP-Grade™ Purified Antibody can be used for 2-5 or 10-20 immunoprecipitations, respectively, at the recommended dilutions.

Application References

(PubMed link indicates BioLegend citation)
  1. Botuyan MV, et al. 2006. Cell. 127:1361. (WB)
RRID
AB_2632896 (BioLegend Cat. No. 827703)
AB_2632844 (BioLegend Cat. No. 827704)

Antigen Details

Structure
Histone proteins H3 and H4 bind to form a tetramer. This tetramer further combines with two H2a-H2b dimers to form the compact Histone octamer core.
Distribution

Histone H4 is ubiquitously expressed in all cells.

Function
Transcription regulation, DNA repair, DNA replication, chromosomal stability, silencing of repetitive DNA and transposons.
Interaction
Various transcription factors.
Biology Area
Cancer Biomarkers, Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
Antigen References

1. Chen HM, et al. 2015. Epigenetics and Dermatology 409.
2. Bhasin M, et al. 2006. J. Comput. Biol. 13:102.
3. Nelson DM, et al. 2016. Genome Biol. 17:158. 

Gene ID
8359 View all products for this Gene ID
UniProt
View information about Histone H4 Trimethyl on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 08.16.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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