- Clone
- 6F8-D9 (See other available formats)
- Other Names
- Histone H4, histone 1, H4a, H4 histone family, member A, H4K20me3
- Isotype
- Mouse IgG1
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of HeLa cells with either A)1:50 dilution of Go-ChIP-Grade™ Purified anti-Histone H4 Trimethyl (Lys20) Antibody (clone 6F8-D9, Cat. No. 827703) or B) equal amount of Purified Mouse IgG1 Isotype Control Antibody (Clone MG1-45, Cat. No. 401401) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human MYOD1 gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin. -
Total lysates (15 µg protein) from Jurkat (lane 1), Raw 264.7 (lane 2) and UMR106 cells (lane 3) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL of purified anti-Histone H4 Trimethyl (Lys20) antibody (clone 6F8-D9). Proteins were visualized using chemiluminescence detection after incubation with a 1:3000 dilution of HRP conjugated anti-Mouse-IgG secondary antibody for anti-Histone H4 Trimethyl (Lys20) (upper panel) or a 1:5000 dilution of Direct-Blot™ HRP anti-β-Actin (clone 2F1-1, lower panel). Lane M: Molecular weight ladder. -
IHC staining of anti-Histone H4 Trimethyl (Lys 20, clone 6F8-D9) on formalin-fixed paraffin-embedded rat brain tissue at 10 µg/ml.
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 827703 | 25 µg | 75 CHF | ||||
| 827704 | 100 µg | 235 CHF | ||||
Histone proteins are classified into core histones (H2A, H2B, H3, H4) and linker histones (H1, H5). Core histones form an octamer, which contains two H2A-H2B dimers and one H3-H4 tetramer. Core histones are predominantly globular except for the unstructured N-terminal tails. Posttranslational modifications, such as acetylation, methylation, phosphorylation, ubiquitination, SUMOylation and ADP-ribosylation occur in histone tails.
Histone modifications induce changes of chromatin structure and thereby affect the accessibility of transcription factors, nuclear proteins and enzymes to genomic DNA, resulting in gene activation or repression. It is known that histone modifications play critical roles in DNA repair, DNA replication, transcription regulation, alternative splicing and chromosome condensation and some diseases including autoimmune diseases and cancers.
Product Details
- Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a synthetic peptide conjugated to KLH containing trimethylated lysine 20 of human Histone H4
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ChIP - Quality tested
IHC-P, WB - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50-1:100 by volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested dilution is 1:100 - 1:500. For Western blotting, the suggested use of this reagent is 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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This antibody is effective in immunohistochemistry (IHC) and Western blotting (WB).
- Additional Product Notes
-
25 µg and 100 µg of Go-ChIP-Grade™ Purified Antibody can be used for 2-5 or 10-20 immunoprecipitations, respectively, at the recommended dilutions.
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Application References
(PubMed link indicates BioLegend citation) -
- Botuyan MV, et al. 2006. Cell. 127:1361. (WB)
- RRID
-
AB_2632896 (BioLegend Cat. No. 827703)
AB_2632844 (BioLegend Cat. No. 827704)
Antigen Details
- Structure
- Histone proteins H3 and H4 bind to form a tetramer. This tetramer further combines with two H2a-H2b dimers to form the compact Histone octamer core.
- Distribution
-
Histone H4 is ubiquitously expressed in all cells.
- Function
- Transcription regulation, DNA repair, DNA replication, chromosomal stability, silencing of repetitive DNA and transposons.
- Interaction
- Various transcription factors.
- Biology Area
- Cancer Biomarkers, Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
- Antigen References
-
1. Chen HM, et al. 2015. Epigenetics and Dermatology 409.
2. Bhasin M, et al. 2006. J. Comput. Biol. 13:102.
3. Nelson DM, et al. 2016. Genome Biol. 17:158. - Gene ID
- 8359 View all products for this Gene ID
- UniProt
- View information about Histone H4 Trimethyl on UniProt.org
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