Go-ChIP-Grade™ Purified anti-HDAC1 Antibody

Pricing & Availability
Clone
10E2/HDAC1 (See other available formats)
Other Names
Histone deacetylase 1, reduced potassium dependency, yeast homolog-like 1
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
1_10E2slashHDAC1_GoChIP_HDAC1_Antibody_050718
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of Jurkat cells with either A)1:100 dilution of Go-ChIP-Grade™ Purified anti-HDAC1 (Clone 10E2/HDAC1, Cat. No. 815103) or B) equal amount of Purified Mouse IgG1, κ Isotype Control Antibody (Clone MG1-45, Cat. No. 401401) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region or α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • 1_10E2slashHDAC1_GoChIP_HDAC1_Antibody_050718
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of Jurkat cells with either A)1:100 dilution of Go-ChIP-Grade™ Purified anti-HDAC1 (Clone 10E2/HDAC1, Cat. No. 815103) or B) equal amount of Purified Mouse IgG1, κ Isotype Control Antibody (Clone MG1-45, Cat. No. 401401) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region or α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • 2_10E2slashHDAC1_PURE_HDAC1_Antibody_WB_3_120117
    Staining of Clone 10E2/HDAC1 on formalin fixed paraffin embedded human liver.
  • 3_10E2slashHDAC1_PURE_HDAC1_Antibody_WB_2_120117
    Total cell lysates (15 µg protein) from HeLa (lanes marked as 1) and NIH3T3 cells (lanes marked as 2) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to a nitrocellulose membrane, and probed with serial dilutions of purified anti-HDAC1 antibody (clone 10E2/HDAC1) as indicated (1:500, 1 µg/mL; 1:1000, 0.5 µg/mL; 1:2000, 0.25 µg/mL; 1:4000, 0.125 µg/mL). Proteins were visualized using chemiluminescence detection after incubation with a 1:3000 dilution of HRP conjugated goat anti-mouse-IgG secondary antibody for the anti-HDAC1 (upper panel) or a 1:5000 dilution of Direct-Blot™ HRP anti-β-Actin (clone 2F1-1, lower panel). Lane M: Molecular weight ladder. Non-specific bands can be detected at high antibody concentrations.
Cat # Size Price Quantity Avail. Save
815103 25 µg 75 CHF
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815104 100 µg 235 CHF
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Description

HDAC1 is a member of class I histone deacetylase (HDACs) family, which modulates the chromatin structure by removing acetyl groups (deacetylation) from the side chain of lysine residues on the N-terminal region of core histones (H2A, H2B, H3 and H4). Generally, histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle programming and developmental process.

HDAC1 is a 65kD nuclear protein, operating in concert with histone acetyltransferases to control core histone acetylation in highly conserved lysine residues. HDAC activity has been associated with transcriptional repression and nucleosomal condensation. HDAC1 deacetylates RELA at residue K310, which results in the inhibition of NF-κB activity. HDAC1-deficient mice were shown to be embryonic lethal, suggesting that HDAC1 plays a crucial role during embryonic development. HDAC1 also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2 MTA2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a peptide sequence derived from the C-terminus of the human HDAC1 protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
IHC-P, WB - Validated

Recommended Usage

Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:100-1:350 by volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested dilution is 1:50 - 1:100. For Western blotting, the suggested use of this reagent is 1:1000-1:4000. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

25 µg and 100 µg of Go-ChIP-Grade™ Purified Antibody can be used for 5-16 or 20-67 immunoprecipitations, respectively, at the recommended dilutions.

RRID
AB_2650673 (BioLegend Cat. No. 815103)
AB_2650659 (BioLegend Cat. No. 815104)

Antigen Details

Distribution

Widely expressed in many cell types; nucleus.

Function
Transcriptional regulation, cell cycle programming, developmental process.
Interaction
Androgen receptor, BCL6, BTG2, BUB1B, BUB1, BUB3, CBFA2T3, CDC20, CDH1, CHD3, CHD4, COUP-TFII, CTBP1, DDX17, DDX5, DNMT3A, DNMT3L, Death-associated protein 6, EED, EVI1, EZH2, FKBP3, GATA1, HMG20B, HSPA4, HUS1, Histone deacetylase 2, Homeobox protein TGIF
Biology Area
Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
Antigen References

1. Chen HM, et al. 2015. Epigenetics and Dermatology. 409:441.
2. Bhaskara S. 2015. Cell Cycle 14:1779.

Gene ID
3065 View all products for this Gene ID
UniProt
View information about HDAC1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 05.07.2018

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