FITC anti-human Lineage Cocktail (CD3, CD14, CD19, CD20, CD56)

Pricing & Availability
UCHT1; HCD14; HIB19; 2H7; HCD56;
Regulatory Status
Mouse IgG1, κ/Mouse IgG2b, κ
Ave. Rating
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Product Citations
Human peripheral blood leukocytes stained with FITC anti-Human Lineage Cocktail (CD3, CD14, CD19, CD20, CD56) and HLA-DR PerCP
  • hlin2cocktail_fitc_1_083110
    Human peripheral blood leukocytes stained with FITC anti-Human Lineage Cocktail (CD3, CD14, CD19, CD20, CD56) and HLA-DR PerCP
  • hlin2cocktail_fitc_2_083110
    Human peripheral blood co-stained with CD11c APC and CD16 PE, gated on the lineage negative/dim and HLA-DR+ population
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348701 50 tests 581 CHF
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This anti-Human Lineage Cocktail is optimized for the detection of human lymphocytes, monocytes, eosinophils, and neutrophils. In combination with other markers, it can be used for studies of dendritic cells (DCs), including DCs that express CD16. This cocktail is composed of CD3, CD14, CD19, CD20, and CD56. CD3 is the antigen mainly found on T cells; CD14 is expressed on monocytes/macrophages and at low levels on neutrophils and eosinophils; CD19 and CD20 are on B cells; CD56 is located on NK cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Product Citations
  1. Rosendahl Huber A, et al. 2022. STAR Protoc. 3:101361. PubMed
  2. Brandsma AM, et al. 2021. Blood Cancer Discov. 2:484. PubMed
  3. de Kanter JK, et al. 2021. Cell Stem Cell. 28:1726. PubMed
  4. Qian P et al. 2018. Cell stem cell. 22(5):740-754 . PubMed
  5. Brandsma AM, et al. 2021. Cancer Discov. . PubMed
  6. Cai D, et al. 2021. Front Mol Biosci. 8:686803. PubMed
  7. Osorio FG et al. 2018. Cell reports. 25(9):2308-2316 . PubMed
  8. Awad MM, et al. 2021. Front Oncol. 11:696512. PubMed
  9. Webb K et al. 2018. Frontiers in immunology. 2.574305556 . PubMed
  10. Hasaart KAL, et al. 2022. iScience. 25:103736. PubMed
  11. Rai S, et al. 2022. Nat Commun. 13:5346. PubMed
  12. Okumura T, et al. 2019. Stem Cell Res Ther. 10:185. PubMed
AB_10644012 (BioLegend Cat. No. 348701)

Antigen Details


CD3 is expressed on all T lymphocytes. CD14 is expressed on monocytes, macrophages, neutrophils, and eosinophils. CD19 and CD20 are expressed on B lymphocytes. CD56 is expressed on activated and resting NK lymphocytes.

Cell Type
Dendritic cells, Eosinophils, Macrophages, Mesenchymal Stem Cells, Monocytes, Neutrophils, T cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules. New Jersey.
2. Olweus J, et al. 1997. P. Natl. Acad. Sci. USA 94:12551.
3. Banchereau J, et al. Eds. 1995. Advances in Experimental Medicine and Biology. New York.
4. Markowicz S, et al. 1990. J. Clin. Invest. 85:955.
5. Freudenthal P, et al. 1990. P. Natl. Acad. Sci. USA 87:7698.
6. Macdonald K, et al. 2002. Blood 100:4512.
7. Piccioli D, et al. 2007. Blood 109:5371.

Gene ID
915 View all products for this Gene ID 930 View all products for this Gene ID 931 View all products for this Gene ID 4684 View all products for this Gene ID 929 View all products for this Gene ID
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Go To Top Version: 2    Revision Date: 07.09.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-human Lineage Cocktail (CD3, CD14, CD19, CD20, CD56)

  • APC anti-human Lineage Cocktail (CD3, CD14, CD19, CD20, CD56)


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