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FITC anti-human CD45RA Antibody

Pricing & Availability
Clone
HI100 (See other available formats)
Regulatory Status
RUO
Workshop
IV N906
Other Names
GP180, L-CA, LCA, LY5, T200, PTPRC
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
HI100
Human peripheral blood lymphocytes stained with HI100 FITC
  • HI100
    Human peripheral blood lymphocytes stained with HI100 FITC
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability Save
304105 25 tests 28 CHF
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304106 100 tests 66 CHF
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304148 100 µg 129 CHF
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Description

CD45RA is a 205-220 kD single chain type I glycoprotein. It is an exon 4 splice variant of the tyrosine phosphatase CD45. The CD45RA isoform is expressed on resting/naïve T cells, medullary thymocytes, B cells and monocytes. CD45RA enhances both T cell receptor and B cell receptor signaling. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
µg sizes: 0.5 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for relevant formats of this clone) include: inhibition of CD45 functions2, immunohistochemical staining of frozen tissue sections3 and formalin-fixed paraffin-embedded tissue sections4, and immunocytochemistry15,16.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Yamada T, et al. 2002. J. Biol. Chem. 277:28830. (WB, Block)
  3. Weninger W, et al. 2003 J. Immunol. 170:4638. (IHC-F)
  4. Imanguli MM, et al. 2009. Blood. 113:3620 (IHC-P)
  5. Roque S, et al. 2007. J. Immunol. 178:8028. (FC) PubMed
  6. Smeltz RB. 2007. J. Immunol. 178:4786. (FC) PubMed
  7. Palendira U, et al. 2008. Blood (FC) PubMed
  8. Kuttruff S, et al. 2009. Blood 113:358. (FC) PubMed
  9. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  10. Alanio C, et al. 2010. Blood 115:3718. (FC) PubMed
  11. Iannello A, et al. 2010. J. Immunol. 184:114. (FC) PubMed
  12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  13. Guereau-de-Arellan M, et al. 2011. Brain. 134:3578. PubMed
  14. Canque B, et al. 2000. Blood 96:3748. (ICC)
  15. Imanguli MM, et al. 2009. Blood 13:3620. (ICC)
  16. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  17. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Iwahori K, et al. 2019. Sci Rep. 2.205555556. PubMed
  2. Cirovic B, et al. 2020. Cell Host & Microbe. 28(2):322-334. PubMed
  3. Ge Y, et al. 2016. Diabetes. 65: 794 - 802. PubMed
  4. Bachmann M, et al. 2012. J Biol Chem. 287:41684. PubMed
  5. Webb LMC, et al. 2021. Aging Cell. 20:e13295. PubMed
  6. Sun J, et al. 2018. Scand J Immunol. 88:e12674. PubMed
  7. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  8. Hess NJ, et al. 2020. J Immunol. 205:272. PubMed
  9. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  10. Weithauser A, et al. 2013. J Am Coll Cardiol. 62:1737. PubMed
  11. Gao Y, et al. 2021. Oncogenesis. 10:62. PubMed
  12. Mori Y, et al. 2015. Proc Natl Acad Sci U S A. 112: 9638-9643. PubMed
  13. Izumi K, et al. 2021. Immunohorizons. 5:687. PubMed
  14. Ramaswamy A, et al. 2021. Immunity. 54(5):1083-1095.e7. PubMed
  15. Kan S, et al. 2020. Int J Oncol. 57:1047. PubMed
  16. Sandborn WJ, et al. 2021. Gastroenterology. 161:1853. PubMed
  17. Toews K, et al. 2020. Mol Carcinog. 724:59. PubMed
  18. Obradovic A, et al. 2021. Cell. 184(11):2988-3005.e16. PubMed
  19. Kuebler P, et al. 2015. Proc Natl Acad Sci U S A. 112: 8379 - 8384. PubMed
  20. Van Egeren D, et al. 2021. Cell Stem Cell. 28(3):514-523.e9. PubMed
  21. Osorio FG et al. 2018. Cell reports. 25(9):2308-2316 . PubMed
  22. Bharath LP, et al. 2020. Cell Metabolism. 32(1):44-55.e6.. PubMed
  23. Arcangeli S, et al. 2022. J Clin Invest. 132: . PubMed
  24. Li Q, et al. 2012. J Leukoc Biol. 91:311. PubMed
  25. Zhu S, et al. 2022. J Oncol. 2022:8724933. PubMed
  26. Su X, et al. 2022. J Transl Med. 20:378. PubMed
  27. Dos Santos JC, et al. 2019. Cell Rep. 28:2659. PubMed
  28. Cesana M, et al. 2018. Cell Stem Cell. 22:575. PubMed
  29. Theobald SJ, et al. 2022. EMBO Mol Med. 14:e15888. PubMed
  30. Szklarski M, et al. 2021. Front Immunol. 12:644548. PubMed
  31. Lima J, et al. 2016. Reprod Sci. 10.1177/1933719116653680. PubMed
  32. Yuan J et al. 2018. Molecular cell. 72(2):380-394 . PubMed
  33. Kubin ME, et al. 2017. Acta Derm Venereol. 97:449. PubMed
  34. Vadrevu KM, et al. 2022. Sci Rep. 12:12038. PubMed
  35. Lancioni C, et al. 2011. Infect Immun. 79:663. PubMed
  36. Rabold K, et al. 2022. Nat Commun. 13:6149. PubMed
  37. Smith-Díaz CC, et al. 2021. Front Oncol. 11:709543. PubMed
  38. Isobe T, et al. 2022. Nat Commun. 13:4501. PubMed
  39. Burke MT, et al. 2017. Transplant Direct. 3:e171. PubMed
  40. Zhao B, et al. 2013. PLoS One. 8:77708. PubMed
  41. Tao L, et al. 2020. Cancers (Basel). 12:00. PubMed
  42. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  43. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  44. Steindor M, et al. 2015. PLoS One. 10:119737. PubMed
  45. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  46. Li M, et al. 2020. Nat Commun. 4051:11. PubMed
RRID
AB_314409 (BioLegend Cat. No. 304105)
AB_314410 (BioLegend Cat. No. 304106)
AB_2564157 (BioLegend Cat. No. 304148)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane (exon 4 splicing of CD45 gene), 205-220 kD
Distribution

B cells, naïve T cells, monocytes

Function
Enhances TCR and BCR signaling
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.

Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45RA on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45RA Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD45RA HI100 FC
Biotin anti-human CD45RA HI100 FC
FITC anti-human CD45RA HI100 FC
PE anti-human CD45RA HI100 FC
PE/Cyanine5 anti-human CD45RA HI100 FC
Purified anti-human CD45RA HI100 FC,CyTOF®,ICC,IHC-P,IHC-F,SB
Alexa Fluor® 488 anti-human CD45RA HI100 FC
Alexa Fluor® 647 anti-human CD45RA HI100 FC,IHC-P
Pacific Blue™ anti-human CD45RA HI100 FC
Alexa Fluor® 700 anti-human CD45RA HI100 FC
PerCP/Cyanine5.5 anti-human CD45RA HI100 FC
PE/Cyanine7 anti-human CD45RA HI100 FC
APC/Cyanine7 anti-human CD45RA HI100 FC
Brilliant Violet 421™ anti-human CD45RA HI100 FC,IHC-P
Brilliant Violet 570™ anti-human CD45RA HI100 FC
Brilliant Violet 605™ anti-human CD45RA HI100 FC
Brilliant Violet 650™ anti-human CD45RA HI100 FC
Brilliant Violet 711™ anti-human CD45RA HI100 FC
Brilliant Violet 785™ anti-human CD45RA HI100 FC
Brilliant Violet 510™ anti-human CD45RA HI100 FC
Purified anti-human CD45RA (Maxpar® Ready) HI100 FC,CyTOF®
PE/Dazzle™ 594 anti-human CD45RA HI100 FC
APC/Fire™ 750 anti-human CD45RA HI100 FC
PerCP anti-human CD45RA HI100 FC
FITC anti-human CD45RA HI100 FC
TotalSeq™-A0063 anti-human CD45RA HI100 PG
Alexa Fluor® 594 anti-human CD45RA HI100 IHC-P
TotalSeq™-B0063 anti-human CD45RA HI100 PG
TotalSeq™-C0063 anti-human CD45RA HI100 PG
Brilliant Violet 750™ anti-human CD45RA HI100 FC
Spark NIR™ 685 anti-human CD45RA HI100 FC
APC anti-human CD45RA HI100 FC
PE/Fire™ 640 anti-human CD45RA HI100 FC
PE/Fire™ 700 anti-human CD45RA Antibody HI100 FC
Spark YG™ 581 anti-human CD45RA HI100 FC
TotalSeq™-D0063 anti-human CD45RA HI100 PG
Spark Violet™ 423 anti-human CD45RA HI100 FC,IHC-P
GMP FITC anti-human CD45RA HI100 FC
PE/Cyanine7 anti-human CD45RA HI100 FC
PE/Dazzle™ 594 anti-human CD45RA HI100 FC
APC/Fire™ 750 anti-human CD45RA HI100 FC
Spark UV™ 387 anti-human CD45RA HI100 FC
GMP APC anti-human CD45RA HI100 FC
Spark Blue™ 550 anti-human CD45RA HI100 FC
GMP PE/Dazzle™ 594 anti-human CD45RA HI100 FC
GMP APC/Fire™ 750 anti-human CD45RA HI100 FC
Go To Top Version: 3    Revision Date: 08.26.2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-human CD45RA

  • Biotin anti-human CD45RA

  • FITC anti-human CD45RA

  • PE anti-human CD45RA

  • PE/Cyanine5 anti-human CD45RA

  • Purified anti-human CD45RA

  • Alexa Fluor® 488 anti-human CD45RA

  • Alexa Fluor® 647 anti-human CD45RA

  • Pacific Blue™ anti-human CD45RA

  • Alexa Fluor® 700 anti-human CD45RA

  • PerCP/Cyanine5.5 anti-human CD45RA

  • PE/Cyanine7 anti-human CD45RA

  • APC/Cyanine7 anti-human CD45RA

  • Brilliant Violet 421™ anti-human CD45RA

  • Brilliant Violet 570™ anti-human CD45RA

  • Brilliant Violet 605™ anti-human CD45RA

  • Brilliant Violet 650™ anti-human CD45RA

  • Brilliant Violet 711™ anti-human CD45RA

  • Brilliant Violet 785™ anti-human CD45RA

  • Brilliant Violet 510™ anti-human CD45RA

  • Purified anti-human CD45RA (Maxpar® Ready)

  • PE/Dazzle™ 594 anti-human CD45RA

  • APC/Fire™ 750 anti-human CD45RA

  • PerCP anti-human CD45RA

  • FITC anti-human CD45RA

  • TotalSeq™-A0063 anti-human CD45RA

  • Alexa Fluor® 594 anti-human CD45RA

  • TotalSeq™-B0063 anti-human CD45RA

  • TotalSeq™-C0063 anti-human CD45RA

  • Brilliant Violet 750™ anti-human CD45RA

  • Spark NIR™ 685 anti-human CD45RA

  • APC anti-human CD45RA

  • PE/Fire™ 640 anti-human CD45RA

  • PE/Fire™ 700 anti-human CD45RA Antibody

  • Spark YG™ 581 anti-human CD45RA

  • TotalSeq™-D0063 anti-human CD45RA

  • Spark Violet™ 423 anti-human CD45RA

  • GMP FITC anti-human CD45RA

  • PE/Cyanine7 anti-human CD45RA

  • PE/Dazzle™ 594 anti-human CD45RA

  • APC/Fire™ 750 anti-human CD45RA

  • Spark UV™ 387 anti-human CD45RA

  • GMP APC anti-human CD45RA

  • Spark Blue™ 550 anti-human CD45RA

  • GMP PE/Dazzle™ 594 anti-human CD45RA

  • GMP APC/Fire™ 750 anti-human CD45RA
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