FITC anti-human CD172a/b (SIRPα/β) Antibody

Pricing & Availability
Clone
148 (See other available formats)
Regulatory Status
RUO
Other Names
SIRPa, SIRPb, SIRPalpha/beta, BIT, SHPS1, MFR, P84, PTPNS1, CD172 antigen-like family member A, CD172 antigen-like family member B
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
148_FITC_110308.jpg
Human peripheral blood granulocytes stained with 148 FITC
  • 148_FITC_110308.jpg
    Human peripheral blood granulocytes stained with 148 FITC
See FITC spectral data
Cat # Size Price Quantity Check Availability Save
337304 100 tests 195,00 CHF
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Description

SIRPs (signal-regulatory proteins) are a family of transmembrane glycoproteins that belong to Ig superfamily. SIRPa (CD172a, SIRPb),(SIRPb1,CD172b), and SIRPg (SIRPb2,CD172g) have been described. CD172a/b molecules are expressed on myeloid cells and neurons. CD172a serves as a substrate of activated receptor tyrosine kinases (RTKs). The interaction of CD172a intracellular domain with SHP-1 and SHP-2 displays negative signaling in the regulation of leukocyte adhesion and transmigration. CD47 (IAP) is an extracellular ligand for CD172a. CD172b is a disulfide-linked homodimer. CD172b has been shown to regulate neutrophil transepithelial migration through the interaction with DAP12 and Syk kinase (through TYROBP).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications include: mAb 148 is specific for CD172a/b, and also stains CD172g transfected cells, but not T-lymphocytes

Application References

(PubMed link indicates BioLegend citation)
  1. Piccio L, et al. 2005. Blood 105:2421
  2. Dietrich J, et al. 2000. J. Immunol. 164:9
RRID
AB_2254620 (BioLegend Cat. No. 337304)

Antigen Details

Structure
Signal-regulatory-protein family, Ig superfamily
Distribution

Monocytes/macrophages, granulocytes, dendritic cells, neurons

Ligand/Receptor
CD172a binds CD47, CD172b interacts with DAP12
Cell Type
Dendritic cells, Granulocytes, Macrophages, Monocytes, Neurons
Biology Area
Cell Biology, Immunology
Molecular Family
CD Molecules
Antigen References

1. Kharitonenkov A, et al. 1997. Nature 386:181.
2. Tomasello E, et al. 2000. Eur. J. Immunol. 30:2147.
3. Seiffert M, et al. 2001. Blood 97:2741.
4. Timms JF, et al. 1998. Mol. Cell Biol. 18:3838.
5. Barclay AN and MH. Brown. 2006. Nat. Rev.Immnuol. 6:457

Gene ID
10326 View all products for this Gene ID 140885 View all products for this Gene ID
UniProt
View information about CD172a/b on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 10.25.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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