ELISA MAX™ Deluxe Set Human PD-L2

Pricing & Availability
Regulatory Status
RUO
Other Names
Human PD-L2, Programmed cell death 1 ligand 2, CD273, PDCD1LG2, B7-DC
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Cat # Size Price Quantity Avail. Save
447604 5 plates 315,00 CHF
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Description

PD-L2 (CD273, PDCD1LG2, B7-DC) and its related protein PD-L1 are ligands for the Programmed Cell Death Protein 1 (PD1). PD-L2 can act as an immune checkpoint molecule by negatively regulating T cell proliferation and cytokine production. However, PD-L2 can also stimulate T cell proliferation through interaction with receptors other than PD1. High level of PD-L2 expression can promote tumor metastasis because of its ability to suppress T cells-mediated anti-tumor activity. Moreover, increased PD-L2 expression might dampen the effectiveness of anti-PD-L1 treatments.

BioLegend’s ELISA MAX™ Deluxe Set is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The kit contains a monoclonal capture antibody and biotinylated monoclonal antibody specific for human PD-L2. BioLegend’s ELISA MAX™ Deluxe Set contains the components necessary for the accurate quantification of natural and recombinant human PD-L2. The set is designed for cost-effective and accurate quantification of human PD-L2 in cell culture supernatant, serum, plasma or other biological fluids. BioLegend’s ELISA MAX™ Deluxe Sets are sensitive, accurate, and robust.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human PD-L2 ELISA MAX™ Capture Antibody (200X)
  • Human PD-L2 ELISA MAX™ Detection Antibody (200X)
  • Human PD-L2 Standard Lyophilized
  • Avidin-HRP (1000X)
  • Substrate Solution F
  • Coating Buffer B (5X)
  • Assay Diluent A (5X)

Product Details

Reactivity
Human
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Sensitivity
0.104 pg/mL
Standard Range
2.0 - 125 pg/mL
Materials Not Included
  • Microwell plates: BioLegend Cat. No. 423501 is recommended
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
  • Stop Solution (e.g. 2N H2SO4) BioLegend Cat. No. 423001 is recommended
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer

Antigen Details

Cell Type
T cells
Biology Area
Adaptive Immunity, Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Immuno-Oncology, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Cytokines/Chemokines, Immune Checkpoint Receptors
Gene ID
NA
UniProt
View information about PD-L2 on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 1    Revision Date: 08.16.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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