DyLight™ 488 Donkey anti-rabbit IgG (minimal x-reactivity) Antibody

Pricing & Availability
Poly4064 (See other available formats)
Regulatory Status
Donkey Polyclonal Ig
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Product Citations
293T cells were stained with anti-Tubulin-γ and secondarily labeled with DyLight™ 488 donkey anti-rabbit IgG (clone Poly4064).
  • Poly4064_D488_100410
    293T cells were stained with anti-Tubulin-γ and secondarily labeled with DyLight™ 488 donkey anti-rabbit IgG (clone Poly4064).
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406404 100 µg 90,00 CHF
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This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
The antibody was purified by affinity chromatography, and conjugated with DyLight™ 488 under optimal conditions.
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

ICC - Quality tested
FC - Reported in the literature, not verified in house

Recommended Usage

It is recommended that the reagent be titrated for optimal performance for each application.

* DyLight 488  has a maximum absorption of 493nm and a maximum emission of 518 nm (similar to Alexa Fluor® 488 or FITC).

Application Notes

This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.

Product Citations
  1. Palacios-Arreola M, et al. 2017. Sci Rep. 10.1038/s41598-017-10135-1. PubMed
  2. Parvatiyar K, et al. 2018. Nat Commun. 9:2770. PubMed
  3. Laugsch M, et al. 2019. Cell Stem Cell. 24:736. PubMed
  4. Suzuki T, et al. 2020. J Clin Invest. 130:799. PubMed
  5. Aronovich A, et al. 2013. Blood. 122:2562. PubMed
  6. Chapman N, et al. 2013. J Immunol. 191:6208. PubMed
  7. Granato A, et al. 2014. J Immunol. 192:5761. PubMed
  8. Chen Y, et al. 2014. Blood. 124:936. PubMed
  9. Beam J, et al. 2015. J Biol Chem. 290: 23371-23384. PubMed
  10. Frank E, et al. 2016. Toxicol Pathol. 10.1177/0192623315620587. PubMed
  11. Zheng Q, et al. 2016. Sci Rep. 6: 33489. PubMed
  12. Liu R, et al. 2017. PLoS One. 10.1371/journal.pone.0170666. PubMed
  13. Tsukamoto S, et al. 2021. Stem Cell Research. 53:102363. PubMed
AB_1575130 (BioLegend Cat. No. 406404)

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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