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Direct-Blot™ HRP anti-V5-tag Antibody

Pricing & Availability
Clone
7/4 (See other available formats)
Regulatory Status
RUO
Other Names
GKPIPNPLLGLDST epitope tag
Isotype
Mouse IgG2b, κ
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Product Citations
publications
7-4_DB-HRP_V5-tag_Antibody_031319
0.5 µL of Recombinant Posi-Tag Epitope Tag Protein (Cat No. 931301) and total cell lysates (15 µg total protein) from untransformed BL21 E. coli cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1:4000 dilution of Direct-Blot™ HRP anti-V5-tag antibody, clone 7/4, overnight at 4°C. Proteins were visualized by chemiluminescence detection. Lane M: Molecular Weight marker.
  • 7-4_DB-HRP_V5-tag_Antibody_031319
    0.5 µL of Recombinant Posi-Tag Epitope Tag Protein (Cat No. 931301) and total cell lysates (15 µg total protein) from untransformed BL21 E. coli cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1:4000 dilution of Direct-Blot™ HRP anti-V5-tag antibody, clone 7/4, overnight at 4°C. Proteins were visualized by chemiluminescence detection. Lane M: Molecular Weight marker.
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680604 25 µL 101 CHF
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680603 100 µL 340 CHF
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Description

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. The V5 tag is a 14 amino acid peptide derived from a small epitope (Pk) on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family. Because of its small size, the addition of a V5 tag sequence (GKPIPNPLLGLDST) to a given gene creates a stable fusion product that does not appear to interfere with the biochemical properties of the protein. Many recombinant proteins have been engineered to express a short sequence derived from the V5 molecule known as V5-tag.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Epitope tag
Reported Reactivity
Species independent
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

RRID
AB_2734532 (BioLegend Cat. No. 680604)
AB_2572008 (BioLegend Cat. No. 680603)

Antigen Details

Structure
14 amino acid peptide (GKPIPNPLLGLDST)
Function
The V5 tag can be used to detect the expression of recombinant proteins in bacteria, yeast, insects, and mammalian cell systems.
Biology Area
Cell Biology
Antigen References

1. Uhlen M, et al. 1990. Meth Enzymol. 185:129.
2. Hanke T, et al. 1995. J. Virol. Methods 53:149.
3. Southern JA, et al. 1991. J. Gen. Virol. 72:1551.
4. Thomas SM, et al. 1988. Cell 54:891.

Gene ID
NA
UniProt
View information about V5-tag on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 09.19.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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