Direct-Blot™ HRP anti-Nestin Antibody

Pricing & Availability
Clone
10C2 (See other available formats)
Other Names
NES
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_10C2_DBHRP_Nestin_Antibody_WB_1_120417
Total lysates (15 µg protein) from HeLa (low expression, negative control) and PC-3 (positive control) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:1000 Direct-Blot™ HRP anti-Nestin antibody, clone 10C2. Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • 1_10C2_DBHRP_Nestin_Antibody_WB_1_120417
    Total lysates (15 µg protein) from HeLa (low expression, negative control) and PC-3 (positive control) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:1000 Direct-Blot™ HRP anti-Nestin antibody, clone 10C2. Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • 2_10C2_DBHRP_Nestin_Antibody_WB_080316
    Total cell lysate from NTERA-2 cells (lane 1, 15 µg) and NF-1 cells (lane 2, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with Direct-Blot™ HRP anti-Nestin antibody (clone 10C2). Proteins were visualized with chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
Cat # Size Price Quantity Avail. Save
656811 100 µl 290 CHF
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Description

Nestin is a class VI intermediate filament protein originally found in neuronal stem cells, and is required for survival and renewal of neural progenitor cells. In mixtures, Nestin forms heterodimers with vimentin or internexin, participating in the remodeling of the cell. Nestin was found to be expressed on vascular endothelial progenitor cells and various neoplasms. It is proposed to be a novel angiogenesis marker and cancer therapeutic target.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Fusion protein
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry1.

Application References

(PubMed link indicates BioLegend citation)
  1. Jozef S, et al. 2012. Biomed. Pap. Med. Fac. 156:135. (IHC)
RRID
AB_2616991 (BioLegend Cat. No. 656811)

Antigen Details

Structure
1621 amino acids with predicted molecular weight of 177 kD. The observed molecular weight is larger than 250 kD.
Distribution

Cytosol

Function
Required for survival and renewal of neural progenitor cells; plays roles in angiogenesis and tumor formation
Interaction
Vimentin, alpha-internexin and FHOD3
Cell Type
Neural Stem Cells
Biology Area
Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Intermediate Filaments
Antigen References

1. Matsuda Y, et al. 2013. World J. Gastroenterol. 19:42.
2. Matsuda Y, et al. 2012. Med. Mol. Morphol. 45:59.
3. Krupkova O Jr, et al. 2010. Neoplasma. 57:291.
4. Hombach-Klonisch S, et al. 2008. Arch. Immunol. Ther. Exp. (Warsz). 56:165.
5. Pilkington GJ, et al. 2005. Cell Prolif. 38:423.

Gene ID
10763 View all products for this Gene ID
UniProt
View information about Nestin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 12.04.2017

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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