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Direct-Blot™ HRP anti-Hsp70 Antibody

Pricing & Availability
Clone
W27 (See other available formats)
Regulatory Status
RUO
Other Names
Heat shock protein 70
Isotype
Mouse IgG2a, λ
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Product Citations
publications
W27_DirectBlotHRP_Hsp70_WB_Image_010616
10 µg of total protein extract from HeLa cells was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP anti-HSP70 (clone W27). Proteins were visualized using chemiluminescence detection.
  • W27_DirectBlotHRP_Hsp70_WB_Image_010616
    10 µg of total protein extract from HeLa cells was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP anti-HSP70 (clone W27). Proteins were visualized using chemiluminescence detection.
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648005 100 µL 340 CHF
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Description

Hsp70 is a member of the Hsp family of highly conserved proteins that play a role in a number of important functions. Their synthesis is increased upon environmental stress. They function by acting as molecular chaperones, facilitating protein refolding and preventing protein aggregation. It has been reported that extracellular Hsp70 also exhibits immunomodulatory effects on innate and acquired immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Hsp70 protein from HeLa cells.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

RRID
AB_2566707 (BioLegend Cat. No. 648005)

Antigen Details

Structure
Hsp proteins are the most conserved proteins present in both prokaryotes and eukaryotes. Human HSp70 contains multiple isoforms. Hsp70s consisit of three structural domains, a 44Kd amino-terminal ATPase domain (NBD), followed by an 18 Kd substrate
Distribution

Ubiquitous, cytoplasmic and nuclear

Function
Molecular chaperrones assisting in the folding of proteins and preventing protein aggregation
Biology Area
Cell Biology, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Trafficking and Clearance
Antigen References

1. Sharma D, et al. 2009. Protein Peptide Lett. 16:571.
2. Johnson JD, et al. 2006. J. Leuk. Biol. 79:425.
3. Bukau B, et al. 1998. Cell 92:351.

Gene ID
3308 View all products for this Gene ID
UniProt
View information about Hsp70 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 04.21.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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