- Clone
- L5 (See other available formats)
- Other Names
- FLAG tag
- Isotype
- Rat IgG2a, λ
- Ave. Rating
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- Product Citations
- publications
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50 ng of recombinant GST protein tagged with the DYKDDDDK peptide was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP anti-DYKDDDDK tag (clone L5). Proteins were visualized using chemiluminescence detection.
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 637312 | 25 µL | 85 CHF | ||||
| 637311 | 100 µL | 290 CHF | ||||
The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins.This unique amino acid sequence allows for specific antibody detection in western blotting, immunoprecipitation, and immunostaining techniques.Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins.
Product DetailsProduct Details
- Reactivity
- DYKDDDDK tag epitope
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- DYKDDDDK-tagged mouse Langerin
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
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WB - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.
25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution. - Application Notes
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The L5 clone has been demonstrated to have 2-8 fold better sensitivity in WB than another commonly used antibody clone, M2.
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Application References
(PubMed link indicates BioLegend citation) -
- Park SH, et al. 2008. J Immunol Methods. 331:27.
- Moon SH, et al. 2010. J. Biol Chem. 285:12935. PubMed
- Sasaki M, et al. 2011. J. Biol Chem. 286:39370. PubMed
- Sonder SU, et al. 2012. J Immunol. 188:5906. PubMed
- Jiang Y, et al. 2013. Int Immunol. 25:235. PubMed
- Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
- Toyo-Oak K, et al. 2014. J Neurosci. 34:12168. PubMed
- Product Citations
- RRID
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AB_2734514 (BioLegend Cat. No. 637312)
AB_2566706 (BioLegend Cat. No. 637311)
Antigen Details
- Biology Area
- Cell Biology
- Antigen References
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1. Einhauer A. 2001. J. Biochem. Biophys. Methods. 49:455.
2. Knappik A and Pluckthun A. 1994. Biotechniques. 17:754. - Gene ID
- NA
- UniProt
- View information about DYKDDDDK on UniProt.org
Related FAQs
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