Direct-Blot™ HRP anti-β-actin Antibody

Pricing & Availability
Clone
2F1-1 (See other available formats)
Other Names
Actin, cytoplasmic 1
Isotype
Mouse IgG2b, κ
Ave. Rating
2 reviews
Product Citations
publications
2F1-1_betaactin_WB_Image_080316
Total cell lysate from HeLa cells (lane 1, 15 µg), NIH-3T3 cells (lane 2, 15 µg), U87-MG (lane 3, 15 µg), mouse brain (lane 4, 15 µg) and rat brain (lane 5, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with Direct-Blot™ HRP anti-β actin antibody (clone 2F1-1). Proteins were visualized using chemiluminescence detection.
  • 2F1-1_betaactin_WB_Image_080316
    Total cell lysate from HeLa cells (lane 1, 15 µg), NIH-3T3 cells (lane 2, 15 µg), U87-MG (lane 3, 15 µg), mouse brain (lane 4, 15 µg) and rat brain (lane 5, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with Direct-Blot™ HRP anti-β actin antibody (clone 2F1-1). Proteins were visualized using chemiluminescence detection.
Cat # Size Price Quantity Avail. Save
643808 25 µl 85 CHF
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643807 100 µl 290 CHF
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Description

β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

The binding of this antibody to its target is sensitive to salt concentration. For consistent results, please use TBS/T buffer for Western blotting that contains 0.15 M NaCl as indicated in the BioLegend recommended protocol

Application References

(PubMed link indicates BioLegend citation)
  1. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  2. Joyce CW, et al. 2006. J. Biol. Chem. 281:33053.
  3. Yanagiya T,et al.2007.Obesity.15:572.
  4. KishidaT,et al. 2007.J. Immunol. 179:8554.
  5. Ouimet M, et al. 2008. Arterisocler Thromb Vasc Biol. 28:1144.
  6. Toltlt LJ,et al. 2008.J. Immunol. 181:2165.
  7. Sawada. T,et al. 2008. J. Biol. Chem. 283:26820.
  8. Ikeda D, et al. 2008. Endocrinology. 149:6037.
  9. Ikeda Y, et al. 2010. Mol Endocrinol. 24:1338. PubMed
  10. Kaieda S, et al. 2010. J. Biol Chem. 285:21478. PubMed
  11. Cambos M, et al. 2011. J. Leukoc. Biol. 89:157. PubMed
Product Citations
  1. Kyle Cottrell, Sergej Djuranovic 2016. PLoS One. 11:e0167877. PubMed
Publication Library
RRID
AB_2734515 (BioLegend Cat. No. 643808)
AB_2566701 (BioLegend Cat. No. 643807)

Antigen Details

Structure
Actin, cytoplasmic 1
Distribution

Ubiquitously expressed in the cytoplasm of all eukaryotic cells

Function
Actins are highly conserved proteins that are involved in cell motility
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microfilaments
Antigen References

1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673.
2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133.
3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687.

Gene ID
11461 View all products for this Gene ID
UniProt
View information about beta-actin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 6    Revision Date: 10.06.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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