Brilliant Violet 650 anti-human CD45RO Antibody anti-CD45RO

Pricing & Availability

Clone: UCHL1 (See other available formats)
Regulatory Status: RUO
Workshop: IV N31
Other Names: CD45RO
Isotype: Mouse IgG2a, κ
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Product Citations: publications

UCHL1_BV650_1_052312 — Human peripheral blood lymphocytes were stained with CD45RA FITC and CD45RO (clone UCHL1) Brilliant Violet 650™ (top) or mouse IgG2a, κ Brilliant Violet 650™ isotype control (bottom).

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304231

25 tests

216 CHF

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304232

100 tests

406 CHF

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Description

CD45RO is a 180 kD single chain membrane glycoprotein. It is a splice variant of tyrosine phosphatase CD45, lacking the A, B, and C determinants. The CD45RO isoform is expressed on activated and memory T cells, some B cell subsets, activated monocytes/macrophages, and granulocytes. CD45RO enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1 and CD22. CD45 isoform expression can change in response to cytokines.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee, Cynomolgus, Common Marmoset

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

IL-2 dependent T cell line, CA1

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

The UCHL1 antibody is commonly used in combination with antibodies against CD45RA to discern memory and naïve T cells. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections⁵ and formalin-fixed paraffin-embedded tissue sections⁴, Western blotting², and immunoprecipitation³.

Application References (PubMed link indicates BioLegend citation)

Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (FC)
Ishii T, et al. 2001. P. Natl. Acad. Sci. USA 98:12138. (WB)
Ponsford M, et al. 2001. Clin. Exp. Immunol. 124:315. (IP)
Yamada M, et al. 1996. Stroke 27:1155. (IHC)
Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immunol. 5:430. (IHC)
Baba N, et al. 2010. Int. Immunol. 22:237. PubMed
Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
Weiss L, et al. 2010. P. Natl. Acad. Sci. USA 107:10632. PubMed
Wu YY, et al. 2007. Infect. Immun. 75:4357. PubMed
Mozaffarian N, et al. 2008. Rheumatology 47:1335. PubMed
Roque S, et al. 2007. J. Immunol. 178:8028. PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Smith SH, et al. 1986. Immunology 58:63. (Immunogen)
Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)

Product Citations

Briceño O, et al. 2016. PLoS One. 11:e0166496. PubMed
Bourdely P, et al. 2020. Immunity. 53(2):335-352. PubMed
Mudd PA, et al. 2022. Cell. 185:603. PubMed
Halkias J, et al. 2019. J Clin Invest. 130:3562. PubMed
Colby DJ, et al. 2018. Nat Med. 24:923. PubMed
Rosato PC, et al. 2019. Nat Commun. 10:567. PubMed
Gamradt S, et al. 2021. iScience. 24:103312. PubMed
Clutton G, et al. 2016. Sci Rep. 6:30749. PubMed
Cortés–Rubio CN, et al. 2019. Clin Epigenetics. 11:134. PubMed

RRID

AB_2561359 (BioLegend Cat. No. 304231)
AB_2563462 (BioLegend Cat. No. 304232)

Antigen Details

Structure: Tyrosine phosphatases, type I transmembrane, 180 kD (isoform of CD45 containing none of the A, B, or C determinants)
Distribution: Activated and memory T cells, B cell subsets, monocytes, macrophages, granulocytes
Function: Enhances TCR and BCR signaling
Ligand/Receptor: CD22
Cell Type: B cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells, Tregs
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.
Gene ID: 5788 View all products for this Gene ID
UniProt: View information about CD45RO on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Certificate of Analysis

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Go To Top Version: 2 Revision Date: 07.23.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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