Brilliant Violet 605™ anti-human IL-4 Antibody

Pricing & Availability
Clone
MP4-25D2 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-4, Ia inducing factor (IaIF), B-cell stimulating factor-1 (BSF-1), Hodgkin's cell growth factor (HCGF), Mast cell growth factor-2 (MCGF-2), Macrophage fusion factor (MFF), T cell growth factor-2 (TCGF-2)
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
MP4-25D2_BV605_IL-4_Antibody_FC_1_071013
PMA+ ionomycin-stimulated human peripheral blood lymphocytes (6 hours, in the presence of monensin) were surface stained with CD3 APC, fixed, permeabilized and then stained with IL-4 (clone MP4-25D2) Brilliant Violet 605™ (top) or rat IgG1, κ Brilliant Violet 605™ isotype control (bottom).
  • MP4-25D2_BV605_IL-4_Antibody_FC_1_071013
    PMA+ ionomycin-stimulated human peripheral blood lymphocytes (6 hours, in the presence of monensin) were surface stained with CD3 APC, fixed, permeabilized and then stained with IL-4 (clone MP4-25D2) Brilliant Violet 605™ (top) or rat IgG1, κ Brilliant Violet 605™ isotype control (bottom).
  • MP4-25D2_BV605_IL-4_Antibody_FC_2_071013
Compare all formats See Brilliant Violet 605™ spectral data
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500827 25 tests 226 CHF
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500828 100 tests 446 CHF
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Description

IL-4 is a pleiotropic cytokine that is produced by activated T cells, mast cells, and basophils. IL-4 elicits many different biological responses but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10, and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Pig, Rhesus
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CHO-expressed, recombinant human IL-4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

ELISA Detection1,3 or ELISPOT Detection4,5: The biotinylated MP4-25D2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 8D4-8 antibody (Cat. No. 500702/500707) as the capture antibody.
Flow Cytometry6,9: The fluorochrome-labeled MP4-25D2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-4 -producing cells within mixed cell populations.
Neutralization1-3: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-4 bioactivity (Cat. No. 500815). The MP4-25D2 antibody can neutralize the bioactivity of natural or recombinant IL-4.

Application References

(PubMed link indicates BioLegend citation)
  1. Chretien I, et al. 1989. J. Immunol. Methods 117:67. (ELISA Detection, Neut)
  2. Ramanathan L, et al. 1993. Biochem. 32:3549. (Neut)
  3. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA Detection, Neut)
  4. Mahanty S, et al. 1992. J. Immunol. 148:3567. (ELISPOT Detection)
  5. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. (ELISPOT Detection)
  6. Prussin C, et al. 1995. J. Immunol. Methods 188:117. (ICFC)
  7. Raqib R, et al. 1995. Infect. Immun. 63:289.
  8. Andersson J, et al. 1994. Immunology 83:16.
  9. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (ICFC) PubMed
  10. Kubota M, et al. 1997. J. Immunol. 158:5321.
  11. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. PubMed
  12. Kroneke MA, et al. 2012. J. Immunol. 188:3734. PubMed
Product Citations
  1. Arlehamn C, et al. 2016. PLoS Pathog. 12: 1005760. PubMed
  2. Bacher P et al. 2019. Cell. 176(6):1340-1355 . PubMed
  3. Gu C, et al. 2021. Front Immunol. 12:678036. PubMed
  4. Harb H, et al. 2021. Immunity. 54(6):1186-1199.e7. PubMed
  5. Faissner S, et al. 2022. Front Immunol. 13:980526. PubMed
RRID
AB_2562311 (BioLegend Cat. No. 500827)
AB_2563879 (BioLegend Cat. No. 500828)

Antigen Details

Structure
Cytokine; 15-19 kD (Mammalian)
Bioactivity
Differentiation of naïve CD4+ T cells to the TH2 type, proliferation/differentiation of activated B cells, expression of class II MHC antigens, and of low affinity IgE receptors in resting B cells
Cell Sources
Mast cells, T cells, bone marrow stromal cells
Cell Targets
B cells, T cells, monocytes, endothelial cells, fibroblasts
Receptors
Heterodimer IL-4Rα (CD124); γ-subunit (CD132) in common with IL-2R, IL-7R, IL-13R, IL-15R
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Boulay J, et al. 1992. Curr. Opin. Immunol. 4:294.
3. Dullens H, et al. 1991. In vivo 5:567.
4. Paul W. 1991. Blood 77:1859.

Regulation
Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
Gene ID
3565 View all products for this Gene ID
UniProt
View information about IL-4 on UniProt.org
Go To Top Version: 1    Revision Date: 08.05.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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