- Clone
- AER-37 (CRA-1) (See other available formats)
- Other Names
- FceRIa, FceRI-a, FceRI-alpha, FceRI alpha, high affinity IgE receptor
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood lymphocytes were stained with CD203c PE and FcεRIα (clone AER-37 (CRA-1)) Brilliant Violet 605™ (top) or mouse IgG2b, κ Brilliant Violet 605™ isotype control (bottom). -
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 334627 | 25 tests | 195 CHF | ||||
| 334628 | 100 tests | 405 CHF | ||||
High affinity IgE receptor (FcεRI) plays a key role in IgE-mediated allergic immune response. FcεRI is a tetrameric receptor complex, which is composed of one α-subunit (FcεRIα), one β-subunit, and two γ-subunits. FcεRIα directly binds IgE with high affinity, while the β- and γ-chains are responsible for mediating intracellular signals. FcεRIα is a 50 kD transmembrane protein with Ig superfamily structure. It is primarily found on mast cells and basophils. Further studies have indicated that FcεRIα is also expressed on many inflammatory cells including cutaneuos Langerhans cells, dendritic cells, monocytes of patients with allergic disorders, platelets, bronchial epithelial cells, eosinophils produced in hypereosinophilic syndrome, and neutrophils from allergy-induced asthma patients.
Product DetailsProduct Details
- Reactivity
- Human, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
- Concentration
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Clone AER-37 (CRA-1) has been reported to bind the receptor even in the presence of IgE.4
-
Application References
(PubMed link indicates BioLegend citation) -
- Yamaguchi M, et al. 1999. J. Immunol. 162:5455.
- Suzukawa M, et al. 2005. Int. Immunol. 17:1249.
- Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
- Yamaguchi M, et al. 1999. J. Immunol. 162:5455.
- RRID
-
AB_2566505 (BioLegend Cat. No. 334627)
AB_2566506 (BioLegend Cat. No. 334628)
Antigen Details
- Structure
- Ig superfamily, 50 kD
- Distribution
-
Mast cells, basophils, cutaneuos Langerhans cells, dendritic cells, and monocytes from the patients with allergic disorders, platelets, bronchial epithelial cells, eosinophils from hypereosinophilic syndrome, neutrophils from allergic asthmatic patients
- Function
- Bind IgE, trigger IgE-mediated allergic response
- Ligand/Receptor
- IgE
- Cell Type
- Basophils, Dendritic cells, Eosinophils, Langerhans cells, Mast cells, Monocytes, Neutrophils
- Biology Area
- Immunology
- Molecular Family
- Fc Receptors
- Antigen References
-
1. Riske F, et al. 1991. J. Biol. Chem. 266:11245
2. Gounni AS, et al. 2001. FASEB J. 15:940.
3. Maurer D, et al. 1996. J. Immunol. 157:607
4. Maurer d, et al. 1994. J. Exp. Med. 179:745
5. Campbell AM, et al. 1998. Am. J. Respir. Cell Mol. Biol. 19:92. - Gene ID
- 2205 View all products for this Gene ID
- UniProt
- View information about Fc epsilon RI alpha on UniProt.org
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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