Loading...

Brilliant Violet 510™ anti-human CD27 Antibody

Pricing & Availability
Clone
O323 (See other available formats)
Regulatory Status
RUO
Workshop
IV T-186
Other Names
S152, T14, TNFRSF7
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
O323_BV510_CD27_Antibody_FC_082512
Human peripheral blood lymphocytes were stained with CD27 (clone O323) Brilliant Violet 510™.
  • O323_BV510_CD27_Antibody_FC_082512
    Human peripheral blood lymphocytes were stained with CD27 (clone O323) Brilliant Violet 510™.
Compare all formats See Brilliant Violet 510™ spectral data
Cat # Size Price Quantity Check Availability Save
302835 25 tests 193 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
302836 100 tests 402 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70 and plays an important role in costimulation of T cell activation, and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-κB and SAPK/JNK activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Squirrel Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Product Citations
  1. Woo MS, et al. 2021. iScience. 24(7):102752. PubMed
  2. Nakano M, et al. 2021. Front Immunol. 12:713225. PubMed
  3. Karim F, et al. 2021. Elife. 10:. PubMed
  4. Pholcharee T, et al. 2021. Nat Commun. 12:1063. PubMed
  5. Turner JS, et al. 2021. Nature. 596:109. PubMed
  6. Tu HA, et al. 2020. Cell Rep Med. 1:100155. PubMed
  7. Abeykoon JP, et al. 2021. Br J Haematol. 193:380. PubMed
  8. Nel I, et al. 2021. Diabetologia. 64(10):2306-2321. PubMed
  9. Rossignol ED, et al. 2021. Cell Reports. 35(8):109167. PubMed
  10. Soh KT, et al. 2020. Cytometry B Clin Cytom. 98:161. PubMed
  11. Mudd PA, et al. 2022. Cell. 185:603. PubMed
  12. Turner JS, et al. 2020. Nature. 586:127. PubMed
  13. Setliff I, et al. 2018. Cell Host Microbe. 23:845. PubMed
  14. Cillo AR, et al. 2021. Cell Rep Med. 2:100476. PubMed
  15. Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed
  16. Salumets A, et al. 2022. Aging Cell. 21:e13607. PubMed
RRID
AB_2561382 (BioLegend Cat. No. 302835)
AB_2562086 (BioLegend Cat. No. 302836)

Antigen Details

Structure
TNF-R superfamily, type I transmembrane glycoprotein, 50-55 kD
Distribution

Medullary thymocytes, T and B cell subsets, NK cells

Function
T cell costimulation
Ligand/Receptor
CD70
Cell Type
B cells, NK cells, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Hintzen R, et al. 1994. Immunol. Today 15:307.
2. Agematsu K, et al. 1995. J. Immunol. 154:3627.

Gene ID
939 View all products for this Gene ID
UniProt
View information about CD27 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-human CD27

  • Biotin anti-human CD27

  • FITC anti-human CD27

  • PE anti-human CD27

  • Purified anti-human CD27

  • Alexa Fluor® 647 anti-human CD27

  • Alexa Fluor® 700 anti-human CD27

  • APC/Cyanine7 anti-human CD27

  • PerCP anti-human CD27

  • PerCP/Cyanine5.5 anti-human CD27

  • Pacific Blue™ anti-human CD27

  • Brilliant Violet 421™ anti-human CD27

  • Brilliant Violet 570™ anti-human CD27

  • Brilliant Violet 650™ anti-human CD27

  • Brilliant Violet 605™ anti-human CD27

  • Brilliant Violet 711™ anti-human CD27

  • Brilliant Violet 785™ anti-human CD27

  • Brilliant Violet 510™ anti-human CD27

  • PE/Cyanine7 anti-human CD27

  • Purified anti-human CD27 (Maxpar® Ready)

  • PE/Dazzle™ 594 anti-human CD27

  • APC/Fire™ 750 anti-human CD27

  • TotalSeq™-A0154 anti-human CD27

  • Brilliant Violet 750™ anti-human CD27

  • TotalSeq™-B0154 anti-human CD27

  • TotalSeq™-C0154 anti-human CD27

  • Spark NIR™ 685 anti-human CD27

  • PE/Fire™ 810 anti-human CD27

  • TotalSeq™-D0154 anti-human CD27

  • APC/Fire™ 810 anti-human CD27

  • PE/Cyanine5 anti-human CD27 Antibody

  • Spark UV™ 387 anti-human CD27

  • Spark Blue™ 515 anti-human CD27
ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account