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Brilliant Violet 421™ anti-mouse CD8b (Ly-3) Antibody

Pricing & Availability
Clone
YTS156.7.7 (See other available formats)
Regulatory Status
RUO
Other Names
Lyt-3, Ly-3
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
a-YTS156dot7dot7_BV421_CD8b_Antibody_022519
C57BL/6 mouse splenocytes were first treated with TruStain FcX™ (anti-mouse CD16/32) Antibody and True-Stain Monocyte Blocker™. Cells were then stained with CD3ε PE and CD8b (clone YTS156.7.7) Brilliant Violet 421™ (left) or Rat IgG2b, κ Brilliant Violet 421™ isotype control (right).
  • a-YTS156dot7dot7_BV421_CD8b_Antibody_022519
    C57BL/6 mouse splenocytes were first treated with TruStain FcX™ (anti-mouse CD16/32) Antibody and True-Stain Monocyte Blocker™. Cells were then stained with CD3ε PE and CD8b (clone YTS156.7.7) Brilliant Violet 421™ (left) or Rat IgG2b, κ Brilliant Violet 421™ isotype control (right).
  • b-YTS156dot7dot7_BV421_CD8b_Antibody_IHCF_060719
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of anti-mouse CD8b (clone YTS156.7.7) Brilliant Violet 421™ (blue), anti-mouse CD4 (clone RM4-5) Alexa Fluor® 647 (red) and anti-mouse B220 (clone RA3-6B2) Alexa Fluor® 488 (green) overnight at 4°C. The image was captured by 10X objective.
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
126629 50 µg 253 CHF
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Description

CD8b is the 32 kD β chain of CD8, also known as Lyt-3 or Ly-3. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. McNical AM, et al. 2007. Eur. J. Immunol. 37:1634.
Product Citations
  1. Chen PM, et al. 2022. Sci Adv. 8:eabo4271. PubMed
RRID
AB_2800620 (BioLegend Cat. No. 126629)

Antigen Details

Structure
Ig superfamily, CD8β chain, 34 kD
Distribution

Majority of thymocytes, cytotoxic T cell subsets

Function
Co-receptor of TCR
Ligand/Receptor
MHC class I
Cell Type
T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1.Barclay A, et al. 1997. The Leukocyte antigen Facts Book Academic Press.
2. Zamoyska R. 1994. Immunity 1:243-246.
3. Ellmeier W, et al. 1999. Annu. Rev Immunol 17:523.
4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503.

Gene ID
12526 View all products for this Gene ID
UniProt
View information about CD8beta on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 2    Revision Date: 06.07.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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