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Brilliant Violet 421™ anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
17A2_BV421_1_062911
C57BL/6 mouse splenocytes were stained with CD19 APC and CD3 (clone 17A2) Brilliant Violet 421™ (top) or rat IgG2b, κ Brilliant Violet 421™ isotype control (bottom).
  • 17A2_BV421_1_062911
    C57BL/6 mouse splenocytes were stained with CD19 APC and CD3 (clone 17A2) Brilliant Violet 421™ (top) or rat IgG2b, κ Brilliant Violet 421™ isotype control (bottom).
  • 17A2_BV421_2_062911
  • 17A2_BV421_CD3_Antibody_IF_012914
    C57BL/6 mouse splenocytes were fixed with 2% paraformaldehyde (PFA), and then stained with 5 µg/ml CD3 (clone 17A2) Brilliant Violet 421™ (blue) and 5 µg/ml CD19 (clone 6D5) Alexa Fluor® 647 (red) for 30 minutes at room temperature. The image was captured by 40X objective.
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
100227 125 µL 176 CHF
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100228 50 µg 242 CHF
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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunocytochemistry using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Kalinina A, et al. 2021. STAR Protoc. 2:100368. PubMed
  2. Schädlich IS, et al. 2022. iScience. 25:104470. PubMed
  3. John S, et al. 2020. Sci Rep. 10:11377. PubMed
  4. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  5. Chukkapalli S, et al. 2015. Pathog Dis. 73:9. PubMed
  6. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  7. Lee H, et al. 2020. Cell Metabolism. 31(4):822-836. PubMed
  8. Chen J, et al. 2022. Nat Commun. 13:6759. PubMed
  9. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  10. Montes de Oca R, et al. 2021. Mol Cancer Ther. 20:1941. PubMed
  11. Almshayakhchi R, et al. 2021. Front Oncol. 11:636977. PubMed
  12. Royzman D, et al. 2022. Front Immunol. 13:1012647. PubMed
  13. Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed
  14. Trenevska I, et al. 2021. PLoS One. 16:e0249967. PubMed
  15. Bertocchi A, et al. 2021. Cancer Cell. 39(5):708-724.e11. PubMed
  16. Chao CC, et al. 2020. Cell. 179(7):1483-1498.e22.. PubMed
  17. Britton GJ et al. 2019. Immunity. 50(1):212-224 . PubMed
  18. Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed
  19. Yu Y, et al. 2022. Nat Commun. 13:6357. PubMed
  20. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  21. Glaser AK, et al. 2019. Nat Commun. 10:2781. PubMed
  22. Jackstadt R, et al. 2019. Cancer Cell. 36:319. PubMed
  23. Cameron G, et al. 2015. J Immunol. 195: 4604 - 4614. PubMed
  24. Telford W, et al. 2017. Cytometry A. 91:314. PubMed
  25. Chen G, et al. 2021. Sci Adv. 7:eabg5686. PubMed
  26. Elahi S, et al. 2020. Stem Cell Res. 43:101710. PubMed
  27. Solaymani-Mohammadi S, et al. 2016. J Leukoc Biol. 99: 475 - 482. PubMed
  28. Chukkapalli S, et al. 2015. PLoS One. 10: 0143291. PubMed
  29. Jin C, et al. 2019. Cell. 176:998. PubMed
  30. Hasegawa T, et al. 2020. Sci Rep. 10:13480. PubMed
  31. Bristow CL, et al. 2021. Front Oncol. 11:739080. PubMed
  32. Hu Z, et al. 2020. PLoS One. 15:e0228339. PubMed
  33. Zhu D, et al. 2017. Stem Cell Res Ther. 0.511805556. PubMed
  34. Cartwright ANR, et al. 2021. Cancer Immunol Res. 9:470. PubMed
  35. Heil J, et al. 2021. Nat Commun. 12:6963. PubMed
  36. Royzman D, et al. 2019. Front Immunol. 10:633. PubMed
  37. Tummala G, et al. 2021. Invest Ophthalmol Vis Sci. 62:41. PubMed
  38. Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
  39. Fleskens V, et al. 2019. Cell Rep. 26:3600. PubMed
  40. Henkle TR, et al. 2021. Cancer Res. 81:4560. PubMed
  41. Chow AK, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. :. PubMed
RRID
AB_10900227 (BioLegend Cat. No. 100227)
AB_2562553 (BioLegend Cat. No. 100228)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-mouse CD3 17A2 FC
PE anti-mouse CD3 17A2 FC
Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Alexa Fluor® 647 anti-mouse CD3 17A2 FC,IHC-F,3D IHC
Alexa Fluor® 488 anti-mouse CD3 17A2 FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse CD3 17A2 FC
Alexa Fluor® 700 anti-mouse CD3 17A2 FC
PerCP/Cyanine5.5 anti-mouse CD3 17A2 FC
PE/Cyanine7 anti-mouse CD3 17A2 FC
APC/Cyanine7 anti-mouse CD3 17A2 FC
Brilliant Violet 421™ anti-mouse CD3 17A2 FC,ICC
Brilliant Violet 570™ anti-mouse CD3 17A2 FC
Brilliant Violet 650™ anti-mouse CD3 17A2 FC
Brilliant Violet 785™ anti-mouse CD3 17A2 FC
Brilliant Violet 510™ anti-mouse CD3 17A2 FC
APC anti-mouse CD3 17A2 FC
Ultra-LEAF™ Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Brilliant Violet 605™ anti-mouse CD3 17A2 FC
Alexa Fluor® 594 anti-mouse CD3 17A2 IHC-F,FC,3D IHC,SB
Brilliant Violet 711™ anti-mouse CD3 17A2 FC
Biotin anti-mouse CD3 17A2 FC,IHC-F
PE/Dazzle™ 594 anti-mouse CD3 17A2 FC
APC/Fire™ 750 anti-mouse CD3 17A2 FC
Brilliant Violet 750™ anti-mouse CD3 17A2 FC
TotalSeq™-A0182 anti-mouse CD3 17A2 PG
TotalSeq™-B0182 anti-mouse CD3 17A2 PG
Spark Blue™ 550 anti-mouse CD3 17A2 FC
Spark NIR™ 685 anti-mouse CD3 17A2 FC
TotalSeq™-C0182 anti-mouse CD3 17A2 PG
APC/Fire™ 810 anti-mouse CD3 17A2 FC
PE/Fire™ 640 anti-mouse CD3 17A2 FC
Spark YG™ 570 anti-mouse CD3 17A2 IHC-F
PE/Fire™ 700 anti-mouse CD3 17A2 FC
PE/Cyanine5 anti-mouse CD3 17A2 FC
Spark Blue™ 574 anti-mouse CD3 Antibody 17A2 FC
Spark Violet™ 423 anti-mouse CD3 17A2 FC
PE/Fire™ 810 anti-mouse CD3 17A2 FC
Spark Red™ 718 anti-mouse CD3 17A2 FC
Spark UV™ 387 anti-mouse CD3 17A2 FC
Go To Top Version: 3    Revision Date: 01.04.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-mouse CD3

  • PE anti-mouse CD3

  • Purified anti-mouse CD3

  • Alexa Fluor® 647 anti-mouse CD3

  • Alexa Fluor® 488 anti-mouse CD3

  • Pacific Blue™ anti-mouse CD3

  • Alexa Fluor® 700 anti-mouse CD3

  • PerCP/Cyanine5.5 anti-mouse CD3

  • PE/Cyanine7 anti-mouse CD3

  • APC/Cyanine7 anti-mouse CD3

  • Brilliant Violet 421™ anti-mouse CD3

  • Brilliant Violet 570™ anti-mouse CD3

  • Brilliant Violet 650™ anti-mouse CD3

  • Brilliant Violet 785™ anti-mouse CD3

  • Brilliant Violet 510™ anti-mouse CD3

  • APC anti-mouse CD3

  • Ultra-LEAF™ Purified anti-mouse CD3

  • Brilliant Violet 605™ anti-mouse CD3

  • Alexa Fluor® 594 anti-mouse CD3

  • Brilliant Violet 711™ anti-mouse CD3

  • Biotin anti-mouse CD3

  • PE/Dazzle™ 594 anti-mouse CD3

  • APC/Fire™ 750 anti-mouse CD3

  • Brilliant Violet 750™ anti-mouse CD3

  • TotalSeq™-A0182 anti-mouse CD3

  • TotalSeq™-B0182 anti-mouse CD3

  • Spark Blue™ 550 anti-mouse CD3

  • Spark NIR™ 685 anti-mouse CD3

  • TotalSeq™-C0182 anti-mouse CD3

  • APC/Fire™ 810 anti-mouse CD3

  • PE/Fire™ 640 anti-mouse CD3

  • Spark YG™ 570 anti-mouse CD3

  • PE/Fire™ 700 anti-mouse CD3

  • PE/Cyanine5 anti-mouse CD3

  • Spark Blue™ 574 anti-mouse CD3 Antibody

  • Spark Violet™ 423 anti-mouse CD3

  • PE/Fire™ 810 anti-mouse CD3

  • Spark Red™ 718 anti-mouse CD3

  • Spark UV™ 387 anti-mouse CD3
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