- Clone
- 17A2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T cell antigen receptor complex, T3
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications

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C57BL/6 mouse splenocytes were stained with CD19 APC and CD3 (clone 17A2) Brilliant Violet 421™ (top) or rat IgG2b, κ Brilliant Violet 421™ isotype control (bottom). -
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C57BL/6 mouse splenocytes were fixed with 2% paraformaldehyde (PFA), and then stained with 5 µg/ml CD3 (clone 17A2) Brilliant Violet 421™ (blue) and 5 µg/ml CD19 (clone 6D5) Alexa Fluor® 647 (red) for 30 minutes at room temperature. The image was captured by 40X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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100227 | 125 µL | 176 CHF | ||||
100228 | 50 µg | 242 CHF |
CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- γδTCR-positive T-T hybridoma D1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- µg sizes: 0.2 mg/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
ICC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunocytochemistry using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
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Violet Laser (405 nm)
- Application Notes
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Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.
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Application References
(PubMed link indicates BioLegend citation) - Product Citations
-
- RRID
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AB_10900227 (BioLegend Cat. No. 100227)
AB_2562553 (BioLegend Cat. No. 100228)
Antigen Details
- Structure
- Ig superfamily, CD3/TCR, 20 kD
- Distribution
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Thymocytes (differentiation dependent), mature T cells, NK-T cells
- Function
- Antigen recognition, TCR signal transduction, T cell activation
- Ligand/Receptor
- Peptide antigen/MHC-complex
- Antigen References
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1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263. - Gene ID
- 12502 View all products for this Gene ID
- UniProt
- View information about CD3 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
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It is lot-specific. On average it ranges between 2-4.
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-mouse CD3
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PE anti-mouse CD3
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Purified anti-mouse CD3
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Alexa Fluor® 647 anti-mouse CD3
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Alexa Fluor® 488 anti-mouse CD3
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Pacific Blue™ anti-mouse CD3
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Alexa Fluor® 700 anti-mouse CD3
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PerCP/Cyanine5.5 anti-mouse CD3
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PE/Cyanine7 anti-mouse CD3
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APC/Cyanine7 anti-mouse CD3
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Brilliant Violet 421™ anti-mouse CD3
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Brilliant Violet 570™ anti-mouse CD3
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Brilliant Violet 650™ anti-mouse CD3
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Brilliant Violet 785™ anti-mouse CD3
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Brilliant Violet 510™ anti-mouse CD3
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APC anti-mouse CD3
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Ultra-LEAF™ Purified anti-mouse CD3
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Brilliant Violet 605™ anti-mouse CD3
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Alexa Fluor® 594 anti-mouse CD3
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Brilliant Violet 711™ anti-mouse CD3
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Biotin anti-mouse CD3
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PE/Dazzle™ 594 anti-mouse CD3
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APC/Fire™ 750 anti-mouse CD3
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Brilliant Violet 750™ anti-mouse CD3
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TotalSeq™-A0182 anti-mouse CD3
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TotalSeq™-B0182 anti-mouse CD3
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Spark Blue™ 550 anti-mouse CD3
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Spark NIR™ 685 anti-mouse CD3
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TotalSeq™-C0182 anti-mouse CD3
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APC/Fire™ 810 anti-mouse CD3
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PE/Fire™ 640 anti-mouse CD3
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Spark YG™ 570 anti-mouse CD3
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PE/Fire™ 700 anti-mouse CD3
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PE/Cyanine5 anti-mouse CD3
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Spark Blue™ 574 anti-mouse CD3 Antibody
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Spark Violet™ 423 anti-mouse CD3
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PE/Fire™ 810 anti-mouse CD3
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Spark Red™ 718 anti-mouse CD3
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Spark UV™ 387 anti-mouse CD3
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