- L138D7 (See other available formats)
- Regulatory Status
- Other Names
- BLR1, GPCR6, MDR-15
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
|Cat #||Size||Price||Quantity Check Availability||Save|
|145511||125 µL||187 CHF|
|145512||500 µL||413 CHF|
CD185 is also known as CXCR5. It is the receptor for chemokine CXCL13/BLC, which is chemotactic for B cells. CXCR5 is expressed on B cells and a subset of T cells in the spleen, neuronal tissue, lymph nodes, and bone marrow. It is important for migration of B cells into the B cell follicles of the spleen and Peyer’s patches. Follicular helper T cells (Tfh) also express CXCR5 and the ability of these cells to migrate to the lymph node is modulated by the balanced expression of CCR7 and CXCR5.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- mCXCR5-transfected cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
Clone L138D7 staining works optimally at room temperature or 4°C. Unlike other chemokine receptor antibodies, avoid using L138D7 at 37°C.
- Product Citations
AB_2562127 (BioLegend Cat. No. 145511)
AB_2562128 (BioLegend Cat. No. 145512)
- 42 kD, seven transmembrane protein
B cells and a subset of T cells in mouse spleen, Tfh cells, also expressed in lymph node, bone marrow, and brain
- B cell migration into follicles, possible regulatory role in B cell differentiation and Burkitt's lymphoma
- Cell Type
- B cells, T cells, Tfh
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
1. Kaiser E, et al. 1993. Eur. J. Immunol. 23:2532.
2. Forster R, et al. 1994. Cell. Mol. Biol. 40:381.
3. Forster R, et al. 1994. Blood 84:830.
4. Forster R, et al. 1996. Cell 87:1037.
5. Wolf I, et al. 1998. J. Biol. Chem. 273:28831.
- Gene ID
- 12145 View all products for this Gene ID
- View information about CD185 on UniProt.org
- What is the F/P ratio range of our BV421™ format antibody reagents?
It is lot-specific. On average it ranges between 2-4.
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD185 (CXCR5)
PE anti-mouse CD185 (CXCR5)
APC anti-mouse CD185 (CXCR5)
PerCP/Cyanine5.5 anti-mouse CD185 (CXCR5)
Biotin anti-mouse CD185 (CXCR5)
Brilliant Violet 421™ anti-mouse CD185 (CXCR5)
Brilliant Violet 605™ anti-mouse CD185 (CXCR5)
PE/Cyanine7 anti-mouse CD185 (CXCR5)
Brilliant Violet 650™ anti-mouse CD185 (CXCR5)
FITC anti-mouse CD185 (CXCR5)
PE/Dazzle™ 594 anti-mouse CD185 (CXCR5)
Brilliant Violet 785™ anti-mouse CD185 (CXCR5)
APC/Cyanine7 anti-mouse CD185 (CXCR5)
Brilliant Violet 711™ anti-mouse CD185 (CXCR5)
Alexa Fluor® 647 anti-mouse CD185 (CXCR5)
APC/Fire™ 750 anti-mouse CD185 (CXCR5)
TotalSeq™-A0846 anti-mouse CD185 (CXCR5)
TotalSeq™-C0846 anti-mouse CD185 (CXCR5)
TotalSeq™-B0846 anti-mouse CD185 (CXCR5) Antibody
Brilliant Violet 750™ anti-mouse CD185 (CXCR5)