Brilliant Violet 421™ anti-human CD169 (Sialoadhesin, Siglec-1) Antibody

Pricing & Availability
7-239 (See other available formats)
Regulatory Status
Other Names
Sialic acid binding Ig-like lectin 1 (Siglec-1)
Mouse IgG1, κ
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Product Citations
IFN-γ and TNF-α-stimulated human monocytes (day-3) were stained with CD169 (Sialoadhesin, Siglec-1, clone 7-239) Brilliant Violet 421™ (filled histogram) or Mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
  • 7-239_BV421_CD169_Antibody_072518
    IFN-γ and TNF-α-stimulated human monocytes (day-3) were stained with CD169 (Sialoadhesin, Siglec-1, clone 7-239) Brilliant Violet 421™ (filled histogram) or Mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
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346017 25 tests 193 CHF
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346018 100 tests 424 CHF
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CD169, also known as Siglec-1 and Sialoadhesin (Sn), is a 210 kD type I single membrane-spanning glycoprotein. It is the largest member of the Siglec family, consisting of 1709 amino acids and belonging to the immunoglobulin superfamily. CD169 is expressed by macrophages and dendritic cells. By its affinity to α2,3-linked sialic acid, it is involved in macrophage binding to different cell types such as granulocytes, monocytes, NK, B and T cells. Several CD169 counter receptors, such as CD227 on human breast cancer cells, CD43 on T cells and CD206 on macrophages, have been reported.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Human Rhinovirus (HRV14) infected, monocyte derived-DCs
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting and inhibition of erythrocyte-rosetting with cells expressing CD169.

Application References

(PubMed link indicates BioLegend citation)
  1. Kirchberger S, et al. 2005. J. Immunol. 175:1145.
  2. Schrauf C, et al. 2009. J. Immunol. 183:4440.
AB_2750267 (BioLegend Cat. No. 346017)
AB_2750268 (BioLegend Cat. No. 346018)

Antigen Details

Type I single membrane-spanning lectin, 1709 amino acids, 210 kD, belongs to the immunoglobulin superfamily

Macrophages, dendritic cells

Adhesion molecule
α-2,3-linked sialic acid
CD227, CD43 and CD206
Mediates sialic-acid dependent binding to granulocytes, monocytes, NK, B and T cells.
Cell Type
B cells, Dendritic cells, Macrophages
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules, Siglec Molecules
Antigen References

1. Xiong YS, et al. 2009. Clin. Biochem. 42:1057.
2. Varki A, et al. 2009. Glycoconj J. 26:231.
3. Rempel H, et al. 2008. PLoS One. 3:e1967.
4. Crocker PR, et al. 2001. Trends Immunol. 22:337.
5. Hartnell A, et al. 2001. Blood 97:288.

Gene ID
6614 View all products for this Gene ID
View information about CD169 on

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 07.25.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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