Brilliant Violet 421™ anti-human CD105 Antibody

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

L-cells transfected with human CD105

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application References

(PubMed link indicates BioLegend citation)

1. Bühring HJ, et al. 1991. Leukemia 5:841.
2. Vogel W, et al. 2002. Haematologica 88:126.
3. Osaka M, et al. 2011. Brain Res. 9:1343. PubMed
4. HonMou O, et al. 2011. Brain. 134:1790. PubMed
5. Herrera MB, et al. 2013. Hepatology. 57:311. PubMed
6. Iohara K, et al. 2014. Exp Gerontol. 52:39. PubMed

Product Citations

1. Li Y, et al. 2020. Front Cell Dev Biol. 8:604021. PubMed

RRID

AB_2562242 (BioLegend Cat. No. 323219)

Antigen Details

Structure

Associates with TGF-ß receptor I and II, homodimeric type I integral membrane protein, 90 kD

Distribution

Endothelial cells, activated monocytes/macrophages, bone marrow stromal cells, hematopoietic stem/progenitor cells

Function

Modulates cellular response to TGF-ß, involved in vascular development and remodelling

Ligand/Receptor

TGF-ß1, TGF-ß3

Cell Type

Endothelial cells, Hematopoietic stem and progenitors, Macrophages, Mesenchymal Stem Cells, Monocytes

Biology Area

Angiogenesis, Cell Biology, Immunology, Stem Cells

Molecular Family

Adhesion Molecules, CD Molecules

Antigen References

1. Mason D, et al. Eds. 2002. Leucocyte Typing VII. Oxford University Press. New York.
2. Pierelli L, et al. 2001. Leuk. Lymphoma 42:1195.

Gene ID

2022 View all products for this Gene ID

UniProt

View information about CD105 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

Certificate of Analysis