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Brilliant Violet 421™ anti-GFAP Antibody

Pricing & Availability
Clone
2E1.E9 (See other available formats)
Regulatory Status
RUO
Other Names
Glial fibrillary acid protein
Isotype
Mouse IgG2b
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Product Citations
publications
1_2E1.E9_BV421_GFAP_Antibody_IHCF_103117
C57BL/6 mouse frozen brain tissue was fixed with 4% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5 % Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the tissue was stained with 1.25 µg/ml of Brilliant Violet 421™ anti-GFAP (clone 2E1.E9, blue) overnight at 4°C. Nuclei were counterstained with DRAQ5™ (red). The image was captured with a 10X objective.
  • 1_2E1.E9_BV421_GFAP_Antibody_IHCF_103117
    C57BL/6 mouse frozen brain tissue was fixed with 4% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5 % Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the tissue was stained with 1.25 µg/ml of Brilliant Violet 421™ anti-GFAP (clone 2E1.E9, blue) overnight at 4°C. Nuclei were counterstained with DRAQ5™ (red). The image was captured with a 10X objective.
  • 2_2E1.E9_BV421_GFAP_Antibody_IF_110217
    Day-three cultured postnatal C57BL/6 mouse brain cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then, the cells were intracellularly stained with 5 µg/ml of anti-GFAP (clone 2E1.E9) Brilliant Violet 421™ in 5% FBS overnight at 4°C. Nuclei were counterstained with DRAQ5™ (blue). The image was captured with a 40X objective.
  • 3_2E1.E9_BV421_GFAP_Antibody_IHCP_103117
    Human paraffin-embedded human cerebellum tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with Brilliant Violet 421™ anti-GFAP Antibody (clone 2E1.E9, blue) at 4°C overnight. The nuclei were counter stained with DRAQ5™ (red). The image was captured with a 10X objective.
Compare all formats See Brilliant Violet 421™ spectral data
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644710 100 µg 435 CHF
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Description

GFAP is expressed exclusively in astrocytes in the central nervous system. The protein is a member of the intermediate filament family of proteins which form networks providing support and strength to cells. This antibody does not cross-react with other intermediate filaments such as vimentin, neurofilament proteins, desmin and others. More than 50 GFAP mutations have been identified to be associated with the Alexander disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Bovine spinal cord homogenate
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested
ICC, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 1.25 - 5.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. McLendon RE and Bigner DD. 1994. Brain Pathol. 4:221.
  2. Liu W, et al. 2011. Proteomics 11:3556. (FC) PubMed
Product Citations
  1. Hill JD, et al. 2019. Brain Behav Immun. 76:165. PubMed
  2. Osaki T, et al. 2020. Nat Protoc. 15:421. PubMed
  3. Fan KQ, et al. 2020. Cell. 179(4):864-879.e19.. PubMed
  4. Sanmarco LM, et al. 2021. Nature. 590:473. PubMed
  5. Rizzo MD, et al. 2019. J Neuroimmunol. 333:576969. PubMed
RRID
AB_2566685 (BioLegend Cat. No. 644710)

Antigen Details

Structure
Around 50 kD
Distribution

Only expressed in astrocytes in the central nervous system.

Function
Together with other proteins to form intermediate filaments which supports astroglial cells. Astroglial cells support and nourish cells in the brain and spinal cord. If brain cells are injured through trauma or disease, astroglial cells react by rapidly prodcing more GFAP protein.
Cell Type
Astrocytes
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
2670 View all products for this Gene ID
UniProt
View information about GFAP on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 3    Revision Date: 02.18.2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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