Biotinylated Recombinant Human CD40 (TNFRSF5)-Fc Chimera (carrier-free)

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Other Names
Tumor necrosis factor receptor superfamily member 5, TNFRSF5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40
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Biotinylated_Recom_Human_CD40_TNFRSF5-Fc_Chimera_CF_1_012521
When recombinant human CD40L (Cat. No. 591702) is immobilized at 1 μg/mL, biotinylated recombinant human CD40-Fc chimera binds in a dose dependent manner with EC50 range of 50-200 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
  • Biotinylated_Recom_Human_CD40_TNFRSF5-Fc_Chimera_CF_1_012521
    When recombinant human CD40L (Cat. No. 591702) is immobilized at 1 μg/mL, biotinylated recombinant human CD40-Fc chimera binds in a dose dependent manner with EC50 range of 50-200 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
  • Biotinylated_Recom_Human_CD40_TNFRSF5-Fc_Chimera_CF_2_012521
    Stability Testing for Biotinylated Recombinant Human CD40-Fc Chimera. Biotinylated recombinant human CD40-Fc Chimera was aliquoted in PBS, 5% glycerol at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4⁰C (Control). The samples were tested in a binding assay with recombinant human CD40L.
Cat # Size Price Quantity Avail. Save
777214 25 µg 190 CHF
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777216 100 µg 450 CHF
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Description

CD40, also known as TNFRSF5, is a type I transmembrane protein that belongs to the TNF receptor family (TNFR). CD40 is expressed on B cells, dendritic cells, monocytes, macrophages, platelets, basal epithelial cells, endothelial cells and a subset of CD34+ hematopoietic progenitors. The interaction between CD40 and its ligand TNFSF5/CD40L leads to the clustering of CD40 and the recruitment of TNFR associated factors (TRAFs), including TRAF1, 2, 3, 5 and 6. TRAF1, 2, 5 and 6 serve as positive regulators while TRAF3 is a negative regulator. CD40/CD40L interaction triggers downstream signaling on both cells, including upregulation of CD40 and cell survival on dendritic cells, upregulation of co-stimulatory molecules, CD80 and CD86 on T cells, and production of inflammatory cytokines. In addition, CD40 regulates B-cell development/maturation, Ig isotype switching and in combination with other signals such as IL-4, promotes proliferation. It has been reported that a transcriptional factor, AKNA, can coordinately regulate the expression of CD40 and CD40L. Dysregulation of CD40 is associated with several diseases, such as hyper-IgM immunodeficiency type 3 (HIGM3) and Hodgkin's disease. Targeting of CD40/CD40L for cancer therapy is being evaluated in clinical trials, including anti-CD40 agonist antibodies and soluble recombinant CD40L. Several isoforms have been identified in human and mice.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human CD40 amino acid Glu21-Arg193 (Accession: #P25942.1), with a linker (GS), a C-terminal human IgG1 (Pro100-Lys330) and an Avi-tag, was expressed in 293E cells. Human CD40-Fc Chimera was site-specifically biotinylated by enzyme BirA.
Molecular Mass
The 421 amino acid recombinant protein has a predicted molecular mass of approximately 47.06 kD. The DTT-reduced and non-reduced protein migrates at approximately 62 kD and 110 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Glu.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE
Formulation
0.22 µm filtered protein solution is in PBS, 5% glycerol.
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
When recombinant human CD40L (Cat. No. 591702) is immobilized at 1 μg/mL, biotinylated recombinant human CD40-Fc chimera binds in a dose-dependent manner with EC50 range of 50-200 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Disulfide linked homodimer
Distribution

B cells, macrophages, monocytes, and dendritic cells

Function
CD40 regulates B-cell development/maturation, Ig isotype switching proliferation. CD40 is also involved in T-cell priming and inflammatory cytokine production.
Interaction
TRAF1, 2, 3, 5, 6
Ligand/Receptor
TNFSF5/CD40L
Bioactivity
Measured by its ability to bind recombinant human CD40L
Antigen References

1. Ridge JP, et al. 1998. Nature 393:474.
2. Schoenberger SP, et al. 1998. Nature 393:480.
3. Bremer E. 2013. ISRN Oncol. 2013:371854.
4. Siddiqa A, et al. 2001. Nature 410: 383.
5. Durandy A, et al. 2005. Immunol. Rev. 203:67.
6. O'Grady JT, et al. 1994. Am. J. Pathol. 144:21.
7. Karaca NE, et al. 2012. Scand. J. Immunol. 76:21.
8. Tone M, et al. 2001. Proc. Natl. Acad. Sci. USA. 98:1751.
9. Eshel D, et al. 2008. Mol. Immunol. 46:250.
10. Hostager BS, et al. 2000. J. Biol. Chem. 275:15392.
11. Elgueta R, et al. 2009. Immunol. Rev. 229:152.

Gene ID
958 View all products for this Gene ID
UniProt
View information about CD40 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 01.25.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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