Biotin anti-mouse Lactadherin Antibody

Pricing & Availability
Clone
Poly5186 (See other available formats)
Regulatory Status
RUO
Other Names
Lactadherin, Milk fat globule-EGF factor 8, MFG-E8, MFGE-8, MFGE8 MFGM, Sperm surface protein SP47, MP47 PAS-6/7, bovine-associated mucoprotein, BA-46, P47
Isotype
Goat Polyclonal Ig
Ave. Rating
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Product Citations
publications
Cat # Size Price Quantity Check Availability Save
518603 50 µg 255,00 CHF
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Description

Lactadherin, a novel adhesion molecule, also known as MFG-E8, is a widely distributed glycoprotein (~50 kD) that has been found in macrophages, dendritic cells, vascular smooth muscle cells, astrocytes, microglia, breast carcinioma cells, and also in breast milk and around blood vessels. Lactadherin contains an N-terminal EGF-like motif with an inserted RGD cell adhesion sequence; it also contains a C-terminal sequence sharing homology with the discoidin family of lectin domains, including the phospholipid-binding domains of blood clotting factors V and VIII. Lactadherin promotes RGD-dependent cell attachment of a variety of cell types, but most notably of cells that express the integrin heterodimer, alpha vβ3. The C1/C2 domain binds specifically to phosphatidylserine. Mounting evidence has shown that Lactadherin plays a far more important role than just protecting the newborn, a function originally identified. Lactadherin is anticoagulant by blocking phosphatidylserine-containing membrane sites for blood coagulation proteins. It is involved in cell death by its ability to recognize specifically phosphatidylserine exposed on apoptotic cells. Lactadherin is also crucial in VEGF-dependent neovascularization and has been identified as an important target for the modulation of neovascularization. In addition, Lactadherin was identified as a novel target for radioimmunotherapy in breast cancer, and is also important in the initiation and/or progression of Alzheimer's disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
293E cells expressed recombinant mouse Lactadherin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by antigen affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.25 - 1.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of mouse Lactadherin recombinant protein ranging from 6000 to 94 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA: The purified poly5186 (Cat. No. 518602) antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5186 antibody (Cat. No. 518603) as the detection antibody.

Note: For testing mouse lactadherin in serum, plasma or cell culture supernatant, BioLegend's ELISA LEGEND MAX™ Kits (Cat. No. 438107 & 438108) are specially developed and recommended.

RRID
AB_2562986 (BioLegend Cat. No. 518603)

Antigen Details

Structure
Glycoprotein, approx. 50 kD
Distribution

Macrophages, dendritic cells, vascular smooth muscle cells, astrocytes, microglia, breast carcinoma cells

Function
Anticoagulant, apoptosis, and neovascularization; also important in the initiation and/or progression of Alzheimer's disease
Interaction
Integrin, phosphatidylethanolamine, phosphatidylserine
Ligand/Receptor
alpha v/β3 and alpha v/β5 receptors
Cell Type
Astrocytes, Dendritic cells, Macrophages, Microglia
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Immunology
Molecular Family
Cytokines/Chemokines
Gene ID
17304 View all products for this Gene ID
UniProt
View information about Lactadherin on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 01.06.2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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