- Poly5186 (See other available formats)
- Regulatory Status
- Other Names
- Lactadherin, Milk fat globule-EGF factor 8, MFG-E8, MFGE-8, MFGE8 MFGM, Sperm surface protein SP47, MP47 PAS-6/7, bovine-associated mucoprotein, BA-46, P47
- Goat Polyclonal Ig
- Ave. Rating
- Submit a Review
- Product Citations
|Cat #||Size||Price||Quantity Check Availability||Save|
|518603||50 µg||255,00 CHF|
Lactadherin, a novel adhesion molecule, also known as MFG-E8, is a widely distributed glycoprotein (~50 kD) that has been found in macrophages, dendritic cells, vascular smooth muscle cells, astrocytes, microglia, breast carcinioma cells, and also in breast milk and around blood vessels. Lactadherin contains an N-terminal EGF-like motif with an inserted RGD cell adhesion sequence; it also contains a C-terminal sequence sharing homology with the discoidin family of lectin domains, including the phospholipid-binding domains of blood clotting factors V and VIII. Lactadherin promotes RGD-dependent cell attachment of a variety of cell types, but most notably of cells that express the integrin heterodimer, alpha vβ3. The C1/C2 domain binds specifically to phosphatidylserine. Mounting evidence has shown that Lactadherin plays a far more important role than just protecting the newborn, a function originally identified. Lactadherin is anticoagulant by blocking phosphatidylserine-containing membrane sites for blood coagulation proteins. It is involved in cell death by its ability to recognize specifically phosphatidylserine exposed on apoptotic cells. Lactadherin is also crucial in VEGF-dependent neovascularization and has been identified as an important target for the modulation of neovascularization. In addition, Lactadherin was identified as a novel target for radioimmunotherapy in breast cancer, and is also important in the initiation and/or progression of Alzheimer's disease.Product Details
- Antibody Type
- Host Species
- 293E cells expressed recombinant mouse Lactadherin
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by antigen affinity chromatography, and conjugated with biotin under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
ELISA Detection - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.25 - 1.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of mouse Lactadherin recombinant protein ranging from 6000 to 94 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA: The purified poly5186 (Cat. No. 518602) antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5186 antibody (Cat. No. 518603) as the detection antibody.
Note: For testing mouse lactadherin in serum, plasma or cell culture supernatant, BioLegend's ELISA LEGEND MAX™ Kits (Cat. No. 438107 & 438108) are specially developed and recommended.
AB_2562986 (BioLegend Cat. No. 518603)
- Glycoprotein, approx. 50 kD
Macrophages, dendritic cells, vascular smooth muscle cells, astrocytes, microglia, breast carcinoma cells
- Anticoagulant, apoptosis, and neovascularization; also important in the initiation and/or progression of Alzheimer's disease
- Integrin, phosphatidylethanolamine, phosphatidylserine
- alpha v/β3 and alpha v/β5 receptors
- Cell Type
- Astrocytes, Dendritic cells, Macrophages, Microglia
- Biology Area
- Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Gene ID
- 17304 View all products for this Gene ID
- View information about Lactadherin on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.