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Biotin anti-mouse CD5 Antibody

Pricing & Availability
Clone
53-7.3 (See other available formats)
Regulatory Status
RUO
Other Names
Lyt-1, Ly-1, T1, Tp67, Ly-12
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
53-7dot3_Biotin_041106
C57BL/6 mouse splenocytes stained with biotinylated 53-7.3, followed by Sav-PE
  • 53-7dot3_Biotin_041106
    C57BL/6 mouse splenocytes stained with biotinylated 53-7.3, followed by Sav-PE
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100603 50 µg 54 CHF
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100604 500 µg 262 CHF
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Description

CD5 is a 67 kD protein, also known as Lyt-1, Ly-1, T1, Tp67, or Ly-12. It is a member of the scavenger receptor cysteine-rich protein superfamily (SRCR) and primarily expressed on thymocytes, T cells, and B-1 cells. Although mature α/β T cells express high levels of CD5, very few γ/δ T cells express this antigen. The interaction of CD5 with CD72, gp35-37, TCR, or BCR is involved in T and B cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, and immunohistochemistry2 of acetone-fixed frozen tissue sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  2. Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (FC, IHC)
  3. Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370.
Product Citations
  1. Wu J, et al. 2022. Nat Commun. 13:7321. PubMed
  2. Sasse C, et al. 2022. Pathogens. 11:. PubMed
  3. Park JG, et al. 2021. iScience. 24(9):102941. PubMed
  4. Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
  5. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  6. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  7. Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
  8. She L, et al. 2021. JCI Insight. 6:e143509. PubMed
  9. He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed
  10. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  11. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  12. Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
  13. Zhang Z, et al. 2019. Haematologica. 4.339583333. PubMed
  14. Galle-Treger L, et al. 2020. J Allergy Clin Immunol. 145:502. PubMed
  15. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  16. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  17. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  18. Rohrbeck L, et al. 2020. Immunol Cell Biol. . PubMed
  19. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  20. Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
  21. Young K, et al. 2021. Cell Stem Cell. . PubMed
RRID
AB_312732 (BioLegend Cat. No. 100603)
AB_312733 (BioLegend Cat. No. 100604)

Antigen Details

Structure
Member of the scavenger receptor cysteine-rich protein superfamily (SRCR), 67 kD
Distribution

Thymocytes, T cells, B-1 cells

Function
Negative regulator of T-B cell interaction
Ligand/Receptor
CD72, gp35-37
Cell Type
B cells, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kipps TJ. 1988. Adv. Immunol. 47:117.
3. Antin JH, et al. 1985. J. Immunol. 136:505.
4. Tarakhovsky A, et al. 1995. Science 269:535.

Gene ID
12507 View all products for this Gene ID
UniProt
View information about CD5 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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