Biotin anti-mouse CD39 Antibody

Pricing & Availability
Clone
5F2 (See other available formats)
Other Names
Entpd1, NTPDase1
Isotype
Mouse IgG1, κ
Ave. Rating
1 reviews
Product Citations
publications
A_5F2_Biotin_111709
CD4+CD25+ (top) and CD4+CD25- cells (bottom) from C57BL/6 lymph node stained with biotinylated 5F2, followed by Sav-PE
  • A_5F2_Biotin_111709
    CD4+CD25+ (top) and CD4+CD25- cells (bottom) from C57BL/6 lymph node stained with biotinylated 5F2, followed by Sav-PE
  • B_5F2_Biotin_111709
Cat # Size Price Quantity Avail. Save
135703 25 µg 100 CHF
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135704 100 µg 220 CHF
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Description

CD39, nucleoside triphosphate diphosphohydrolase-1 (NTPDase 1), is an ectoenzyme that degrades ATP to AMP. It is a member of ectonucleoside triphosphate dihydrolases (E-NTPDases) which are involved in regulation of extracellular nucleotide catabolism and controlling the extracellular nucleoside triphosphate pool (NTP). CD39 is the dominant member of this family in the immune system and involved in suppression of inflammation and control of platelet activation. CD39 is expressed on B cells, dendritic cells, and a subset of T cells including regulatory T cells and memory T cells. The coordinated expression of CD39/CD73 on Tregs and the adenosine A2A receptor on activated T effector cells generates immunosuppressive loops. In human studies, it has been reported that CD4+CD25-CD39+ T cells are T inducers.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant mCD39-Ig, mCD39-expressing pcDNA3.1 plasmid, and B cells (CD39+)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
WB, ELISA - Reported in the literature

Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References

(PubMed link indicates BioLegend citation)
  1. Zhou Q, et al. 2009. Am J. Transplant. 9(10):2303
RRID
AB_2099921 (BioLegend Cat. No. 135703)
AB_2099920 (BioLegend Cat. No. 135704)

Antigen Details

Structure
A member of ectonucleoside triphosphate dihydrolases (E-NTPDases), an ectoenzyme that degrades ATP to AMP.
Distribution
CD39 is expressed on B cells, dendritic cells, and a subset of T cells
Function
CD39 is involved in suppression of inflammation and control of platelet activation. Coordinated expression of CD39/CD73 on Tregs and the adenosine A2A receptor on activated T effector cells generates immunosuppressive loops.
Cell Type
B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
1. Borsellino G et al. 2007. Blood 110(4):1225
2. Deaglio S et al. 2007. J. Exp. Med. 204(6):1257
3. Bynoe MS. et al. 2008. Trends Immunol. 29(3):99
4. Ndhlovu LC. et al. Eur. J. Immunol. 2009 Oct 28. [Epub ahead of print]
Gene ID
12495 View all products for this Gene ID
UniProt
View information about CD39 on UniProt.org

Reviews

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Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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